Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-744
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Western Blot (WB) | 1:1,000-1:10,000 |
Immunocytochemistry (ICC/IF) | 1:10-1:100 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 117 publications below |
Flow Cytometry (Flow) | See 1 publications below |
Immunohistochemistry (IHC) | See 3 publications below |
Immunocytochemistry (ICC/IF) | See 4 publications below |
ELISA (ELISA) | See 1 publications below |
ChIP assay (ChIP) | See 1 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 3 publications below |
Species reactivity | Bovine, Fruit fly, Human, Mouse, Sheep, Plant, Rat, Xenopus, Yeast, Zebrafish |
Published species | Bacteria, Bovine, Fruit fly, Goat, Human, Insect, Mouse, Pig, Plant, Rat, Xenopus, Yeast |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | mAbGEa |
Immunogen | Purified Arabidopsis actin protein. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Storage buffer | ascites |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2223496 |
MA1-744 detects actin protein in human, mouse, rat, bovine, sheep, drosophila, zebra fish (Danio rerio), yeast, arabidopsis and xenopus samples. This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.
MA1-744 has successfully been used in Western blot and ELISA procedures. By Western blot, this antibody detects a 45 kDa protein representing actin.
The MA1-744 immunogen is purified actin protein from Arabidopsis.
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5C actin; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; Act5C-PE; ACTB; actin; actin 5 C; actin 5c; actin A1; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; Actin-5C; actin-like protein; actin5C; alpha 1 skeletal muscle actin; alpha actin 2; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; anon-fast-evolving-2D2; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cellular cytoskeletal beta-actin; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; CG4027-PE; Cytoplasmic beta-actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; Dm-actin5C; epididymis luminal protein 176; F-actin; G-actin; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RE02927p; RP5-1068B5.2; sma; smooth muscle alpha-actin; Smooth muscle gamma-actin
Gene Aliases: 0610041G09Rik; A; a-SMA; A4V404_DROME; AA959943; AAT6; Ac5C; ACT; act 5C; Act-4; Act-5C; ACT1_DROME; Act4; Act5; ACT5C; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actin; Actin/BAP47; Actin5C; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; anon-EST:fe2D2; ArpA; ASD5; ASMA; BAP47; beta-actin; beta-actin/Bap47; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CG4027; chrX:5748184..5748304; CMD1R; CMH11; cyt5C; DFNA20; DFNA26; Dmel\CG4027; Dmel_CG4027; E430023M04Rik; E51; F-actin; G-actin; GIG46; HEL-176; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0177; l(1)G0245; l(1)G0330; l(1)G0420; l(1)G0486; LVNC4; M32055; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; T11; VSCM; wu:fb63d03; XELAEV_18029136mg; XELAEV_18045052mg
UniProt ID: (Human) P68133, (Bovine) P68138, (Bovine) P62739, (Human) P04108, (Bovine) P60712, (Human) Q96B34, (Sheep) P60713, (Human) P68032, (Bovine) Q3ZC07, (Human) P63261, (Bovine) P14104, (Bovine) Q5E9B5, (Human) P63267, (Fruit fly) P10987, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269, (Xenopus) O93400
Entrez Gene ID: (Human) 58, (Bovine) 281592, (Sheep) 101107662, (Bovine) 515610, (Human) 59, (Sheep) 101121051, (Bovine) 280979, (Human) 60, (Sheep) 443052, (Human) 70, (Bovine) 533219, (Sheep) 101111554, (Human) 71, (Bovine) 404122, (Sheep) 443340, (Sheep) 101114256, (Bovine) 281595, (Human) 72, (Fruit fly) 31521, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Rat) 29275, (Mouse) 11464, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365, (Xenopus) 432314, (Zebrafish) 322509, (Xenopus) 398459
Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein
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