Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-450
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:1,000 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 17 publications below |
Immunoprecipitation (IP) | See 3 publications below |
Species reactivity | Human |
Published species | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Fusion protein containing residues 400-460 of the human ATR protein. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 4 mg/mL |
Purification | Affinity chromatography |
Storage buffer | PBS |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_325822 |
PA1-450 detects ATR (Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein) from human cells.
PA1-450 has been successfully used in Western blot immunofluoresence and immunoprecipitation procedures. By Western blot, this antibody detects an ~305 kDa protein representing ATR in lysate from UV irradiated K562 cells.
PA1-450 antigen is a fusion protein corresponding to amino acid residues 400-460 from human ATR.
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2310008J16Rik; 2810405N18Rik; Ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8
Gene Aliases: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1
UniProt ID: (Human) Q59HB2
Entrez Gene ID: (Human) 545
Molecular Function:
non-receptor serine/threonine protein kinase
protein modifying enzyme
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.