Catalog # MA3-923
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ChIP assay (ChIP) | Assay-dependent |
Flow Cytometry (Flow) | 1/50 |
Immunohistochemistry (Frozen) (IHC (F)) | 1:2,000 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:4,000 |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Immunohistochemistry (IHC) | See 36 publications below |
Western Blot (WB) | See 4 publications below |
Flow Cytometry (Flow) | See 3 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 2 publications below |
Immunohistochemistry (Frozen) (IHC (F)) | See 1 publications below |
Immunocytochemistry (ICC/IF) | See 11 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Species reactivity | Bovine, Dog, Human, Mustelid, Mouse, Pig, Rabbit, Rat |
Published species | Bovine, Guinea pig, Human, Mouse, Rabbit, Rat, Rodent |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | 2G11 |
Immunogen | Purified 34 kDa core peptide from deglycosylated hog gastric microsomes. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Storage buffer | PBS, ascites |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2227726 |
MA3-923 detects the beta-subunit of hydrogen/potassium ATPase from bovine, human, canine, porcine, rabbit, mouse, ferret, and rat tissues.
MA3-923 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation procedures (ChIP). By Western blot, this antibody detects various forms of the beta-subunit, including a 60 to 80 kDa glycosylated protein, a 52 kDa beta-subunit precursor, and the 34 kDa core peptide from rabbit stomach extracts. Immunohistochemical staining of hydrogen/potassium ATPase in rabbit gastric glands with MA3-923 results in specific staining of parietal cells.
MA3-923 has been shown to inhibit the enzymatic activity of the hydrogen/potassium ATPase and to alter the affinity of the cytoplasmic potassium binding site.
The MA3-923 antigen is purified 34 kDa core peptide from deglycosylated hog gastric microsomes. This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.
The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATPase H+/K+ transporting beta subunit; ATPase H+K+-transporting beta / (gastric HK-ATPase beta subunit) defined by SSR; ATPase, H+,K+-transporting, beta / (gastric H,K-ATPase beta subunit), defined by SSR; ATPase, H+/K+ exchanging, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide, gastric specific; Gastric H(+)/K(+) ATPase subunit beta; gastric H+/K+ ATPase beta subunit; gastric H+/K+-ATPase beta subunit; gastric hydrogen-potassium ATPase, beta; gp60-90; H+,K+-ATPase; H+/K+ ATPase beta; H+/K+ ATPase beta subunit; H+/K+-ATPase beta; H+/K+-ATPase beta subunit; H,K-ATPase-Beta; H.K-ATPase, beta subunit; potassium-transporting ATPase beta chain; Potassium-transporting ATPase subunit beta; Proton pump beta chain
Gene Aliases: ATP4B; ATP6B; AV080843; S76401S1
UniProt ID: (Pig) P18434, (Rabbit) P18597, (Human) P51164, (Rat) P18598, (Mouse) P50992
Entrez Gene ID: (Pig) 397131, (Rabbit) 100009125, (Human) 496, (Bovine) 614308, (Dog) 404020, (Rat) 24217, (Mouse) 11945
Molecular Function:
primary active transporter
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