Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA3-930
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:1,000-1:10,000 |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 47 publications below |
Immunohistochemistry (IHC) | See 2 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 2 publications below |
Immunocytochemistry (ICC/IF) | See 3 publications below |
Species reactivity | Dog, Human, Mouse, Sheep, Pig |
Published species | Dog, Fruit fly, Human, Invertebrate, Mouse, Sheep |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Clone | M17-P5-F11 |
Immunogen | Purified lamb kidney beta sodium/potassium ATPase. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Protein A |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2060990 |
MA3-930 detects sodium/potassium ATPase beta 1 from mouse, human, sheep, canine, and pig tissues. This antibody does not recognize the beta 1 or beta 2 subunit in rat.
MA3-930 has been successfully used in Western blot and immunohistochemistry and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein in liver samples and ~55 kDa in kidney samples representing beta sodium/potassium ATPase in sheep liver extract. Immunohistochemical staining of the sodium/potassium ATPase with MA3-930 yields a pattern consistent with plasma membrane localization.
The MA3-930 antigen is purified lamb kidney sodium/potassium ATPase beta. This antibody recognizes and epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adenosinetriphosphatase; ATPase beta subunit; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; MGC1798; Na+, K+-ATPase; Na+, K+-ATPase beta-subunit; Na+/K+ ATPase Beta; Na, K-ATPase beta-1 polypeptide; OTTHUMP00000032537; OTTHUMP00000032538; Sodium Potassium ATPase; sodium pump subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium/potassium ATPase beta subunit; sodium/potassium-dependent ATPase beta-1 subunit; Sodium/potassium-dependent ATPase subunit beta-1; sodium/potassium-transporting ATPase beta-1 chain; Sodium/potassium-transporting ATPase subunit beta-1; unnamed protein product
Gene Aliases: ATP1B; ATP1B1; Atp4b; Atpb; Atpb-1; NKbeta1
UniProt ID: (Sheep) P05028, (Pig) P05027, (Human) P05026, (Mouse) P14094
Entrez Gene ID: (Sheep) 443384, (Dog) 403966, (Pig) 396898, (Human) 481, (Mouse) 11931
Molecular Function:
primary active transporter
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