Invitrogen

Manufacturer Code:PA535152

ALDH1A1 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

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Catalog # PIPA535152

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ALDH1A1 Rabbit [PA535152] — Immunofluorescent analysis of ALDH1A1 showing staining in the cytoplasm of HepG2 cells using an ALDH1A1 polyclonal antibody (Product ## PA5-35152) followed by detection using a fluorescent conjugated secondary antibody (green) (1:1000, 1h). Nuclei were stained with Hoechst 33342 (blue) (10 ug/ml, 5 min).

ALDH1A1 Antibody (PA5-35152) in IF — Immunofluorescence analysis of ALDH1A1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ALDH1A1 Polyclonal Antibody (Product ## PA5-35152) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary

ALDH1A1 Antibody (PA5-35152) in WB — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), A-431 (Lane 3), MCF7 (Lane 4), HT-29 (Lane 5), COLO 205 (Lane 6), tissue extracts of Mouse Liver (Lane 7), Rat Liver (Lane 8) and Mouse Skin (Lane 9). The blot was probed with Anti-ALDH1A1 Polyclonal Antibody (Product ## PA5-35152, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product ## A270

Product Details

TESTED APPLICATIONS	DILUTION
Flow Cytometry (Flow)	Assay dependent
Immunocytochemistry (ICC)	Assay dependent
Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (Paraffin) (IHC (P))	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Many
Host / Isotype	Rabbit
Class	Polyclonal
Type	Antibody
Immunogen	human ALDH1A1 recombit protein
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	-20&deg C Avoid Freeze/Thaw Cycles

Product Specific Information

Rabbit Polyclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: acetaldehyde dehydrogenase 1; alcohol dehydrogenase family 1, subfamily A1; alcohol dehydrogenase family 1, subfamily A2; aldehyde dehydrogenase 1 family, member A1; aldehyde dehydrogenase 1, liver cytosolic (class 1); aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase 1, subfamily A1; aldehyde dehydrogenase 1A1; aldehyde dehydrogenase 2; Aldehyde dehydrogenase family 1 member A1; aldehyde dehydrogenase family 1, member A1; aldehyde dehydrogenase family 1, subfamily A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH-E1; ALDH1A1 ALDC ALDH1 PUMB1 Retinal dehydrogenase 1 ALDH-E1 ALHDII Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase cytosolic; ALHDII; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; RALDH 1; ralDH1; Retinal dehydrogenase 1; retinaldehyde dehydrogenase 1

Gene Aliases: Ahd-2; Ahd2; ALDC; Aldh; ALDH-E1; ALDH1; ALDH11; ALDH1A1; Aldh1a2; Aldh2; E1; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1

UniProt ID: (Human) P00352, (Mouse) P24549, (Rat) P51647

Entrez Gene ID: (Human) 216, (Mouse) 11668, (Rat) 24188

Molecular Function: Blue Arrow Icon dehydrogenase oxidoreductase

Function(s)

ethanol oxidation cellular aldehyde metabolic process retinol metabolic process positive regulation of GTPase activity fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate optic cup morphogenesis involved in camera-type eye development retinoic acid biosynthetic process response to oxidative stress metabolic process response to drug retinoic acid metabolic process 9-cis-retinoic acid biosynthetic process 9-cis-retinoic acid metabolic process positive regulation of apoptotic process response to ethanol embryonic eye morphogenesis protein homotetramerization oxidation-reduction process kidney development liver development midgut development response to organic cyclic compound response to estradiol response to retinoic acid estrous cycle

Process(es)

retinal dehydrogenase activity
aldehyde dehydrogenase (NAD) activity
GTPase activator activity
androgen binding
benzaldehyde dehydrogenase (NAD+) activity
3-chloroallyl aldehyde dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
identical protein binding

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ALDH1A1 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

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ALDH1A1 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

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Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

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