Invitrogen

Manufacturer Code:PA511537

ALDH1A1 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

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ALDH1A1 Rabbit [PA511537] — Immunofluorescent analysis using an ALDH1A1 polyclonal antibody (Product ## PA5-11537) in HeLa cells. 0.025 mg/ml primary antibody was followed by fluor dye-conjugated donkey anti-rabbit lgG (H+L) (orange). Blue counterstaining is DAPI.

ALDH1A1 Antibody (PA5-11537) in RE — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Expression of ALDH1A1 Polyclonal Antibody was not observed specifically in A-431 and MCF7 using Anti-ALDH1A1 Polyclonal Antibody (Product ## PA5-11537) in Western Blot.

ALDH1A1 Antibody (PA5-11537) in WB — Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A-431 (Lane 2), MCF7 (Lane 3), PC-3 (Lane 4), MDA-MB-231 (Lane 5) and tissue extract of Mouse Liver (Lane 6). The blot was probed with Anti-ALDH1A1 Polyclonal Antibody (Product ## PA5-11537, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product ## A27036, 0.25 µg/ml, 1:4000 dilution). A 55 kDa band corr

Product Details

TESTED APPLICATIONS	DILUTION
Flow Cytometry (Flow)	Assay dependent
Immunocytochemistry (ICC)	Assay dependent
Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (Paraffin) (IHC (P))	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Many
Host / Isotype	Rabbit
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 302-331 amino acids from the central region of human ALDH1A1
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	-20&deg C Avoid Freeze/Thaw Cycles

Product Specific Information

Rabbit Polyclonal Antibody

For Research Use Only

Bioinformatics

Protein Aliases: acetaldehyde dehydrogenase 1; alcohol dehydrogenase family 1, subfamily A1; alcohol dehydrogenase family 1, subfamily A2; aldehyde dehydrogenase 1 family, member A1; aldehyde dehydrogenase 1, liver cytosolic (class 1); aldehyde dehydrogenase 1, soluble; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH-E1; ALHDII; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; RALDH 1; RalDH1 RALDH 1 Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase cytosolic ALHDII ALDH-E1; Retinal dehydrogenase 1; retinaldehyde dehydrogenase 1

Gene Aliases: Ahd-2; Ahd2; ALDC; ALDH-E1; ALDH1; ALDH11; ALDH1A1; Aldh1a2; E1; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1

UniProt ID: (Human) P00352, (Mouse) P24549

Entrez Gene ID: (Human) 216, (Mouse) 11668

Molecular Function: Blue Arrow Icon dehydrogenase oxidoreductase

Function(s)

ethanol oxidation cellular aldehyde metabolic process retinol metabolic process positive regulation of GTPase activity fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate optic cup morphogenesis involved in camera-type eye development retinoic acid biosynthetic process response to oxidative stress metabolic process response to drug retinoic acid metabolic process 9-cis-retinoic acid biosynthetic process 9-cis-retinoic acid metabolic process positive regulation of apoptotic process response to ethanol embryonic eye morphogenesis protein homotetramerization oxidation-reduction process

Process(es)

retinal dehydrogenase activity
aldehyde dehydrogenase (NAD) activity
GTPase activator activity
androgen binding
benzaldehyde dehydrogenase (NAD+) activity
3-chloroallyl aldehyde dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
identical protein binding

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HRP Anti-Rabbit Secondary Antibodies (100)

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ALDH1A1 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

Product Details

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Bioinformatics

Suggested Secondary Antibodies

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Material safety data sheets (MSDS)

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ALDH1A1 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

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Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Your items have has been added!