Catalog # PA1-042
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:20-1:200 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:1,000 |
Immunocytochemistry (ICC/IF) | 1:10-1:100 |
PUBLISHED APPLICATIONS | |
---|---|
Immunohistochemistry (IHC) | See 3 publications below |
in situ PLA (PLA) | See 1 publications below |
Western Blot (WB) | See 4 publications below |
Species reactivity | Dog, Human, Mouse, Rat |
Published species | Dog, Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 0.69 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2257858 |
PA1-042 detects adenosine receptor A2a (A2aAR) in human, rat, mouse and canine tissues. This antibody does not detect other AR subtypes.
PA1-042 has been successfully used in Western blot, ICC/IF, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~45 kDa protein from canine striatum representing A2aAR. Immunohistochemical staining of A2aAR in human hippocampus with PA1-042 yields a pattern consistent with plasma membrane staining.
The PA1-042 immunogen is a synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR.
ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: A2a receptor; Adenosine A2a-receptor; Adenosine receptor A2a; adenosine receptor subtype A2a; hA2aR; unnamed protein product
Gene Aliases: A2AAR; A2aR; AA2AR; ADENO; ADORA2; ADORA2A; Adora2l1; RDC8
UniProt ID: (Human) P29274, (Dog) P11617, (Mouse) Q60613, (Rat) P30543
Entrez Gene ID: (Human) 135, (Dog) 403960, (Mouse) 11540, (Rat) 25369
Molecular Function:
G-protein coupled receptor
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