Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-81891
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 1:10 |
Western Blot (WB) | 1:250 |
Immunocytochemistry (ICC/IF) | Assay-dependent |
PUBLISHED APPLICATIONS | |
---|---|
Flow Cytometry (Flow) | See 1 publications below |
Immunocytochemistry (ICC/IF) | See 1 publications below |
Western Blot (WB) | See 1 publications below |
Species reactivity | Tag |
Published species | Tag |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | AD1.1.10 |
Immunogen | PAX6 transcription factor linked to histidine tag. |
Conjugate | FITC |
Form | Liquid |
Concentration | 0.1 mg/mL |
Purification | Protein G |
Storage buffer | PBS, pH 7.4, with 1% BSA |
Contains | 0.09% sodium azide |
Storage conditions | 4° C |
RRID | AB_931255 |
MA1-81891 recognizes proteins containing the motiff H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts aginst all His-tagged proteins tested so far.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; His Tag; Histidine Tag
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