Invitrogen
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA5-40097
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | 1:2,800 |
Immunoprecipitation (IP) | 3.5 µg |
Peptide array (Array) | 1:1,000 |
Species reactivity | Human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Human KLH-conjugated 5-hydroxymethylcytosine. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 3.5 mg/mL |
Purification | Protein G |
Storage buffer | PBS, pH 7.4 |
Contains | 0.05% ProClin 300, 0.05% sodium azide |
Storage conditions | -20°C or -80°C if preferred |
RRID | AB_2610671 |
5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5-hmc; 5-HydroxymethylCytidine; 5hmc; C5H7N3O2
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