Catalog # PA1-30675
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Dot blot (DB) | Assay-dependent |
Flow Cytometry (Flow) | Assay-dependent |
Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL |
Immunoprecipitation (IP) | 25-100 µg/mL |
Western Blot (WB) | 1:1,000 |
Immunocytochemistry (ICC/IF) | 5 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Immunohistochemistry (IHC) | See 1 publications below |
Species reactivity | Chemical |
Published species | Not Applicable |
Host / Isotype | Sheep / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | 5-methyl cytosine molecule conjugated to KLH. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 3 mg/mL |
Purification | purified |
Storage buffer | 0.15M PBS |
Contains | no preservative |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_1957442 |
The use of 7mM NaOH in the IP is crutial.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5-mC; 5-meC; 5-Methyl-cytosine; 5mc; C5H7NO3
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