Test
Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.
Catalyze the hydrolysis of inorganic pyrophosphate to two orthophosphates.
Components | 100mM Tris-HCl 100, 50mM magnesium chloride, 5mM dithiothreitol, and 5mM sodium orthovanadate, pH 7.4. |
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Content And Storage | Ambient temperatures |
Form | Liquid |
Product Type | Kinase buffer I (5X) |
For cellular and molecular biology applications
Components | 200mM Tris, 200mM NaCl, and 40mM magnesium chloride at pH 9.5 |
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Content And Storage | Keep cold |
Form | Liquid |
Product Type | Alkaline Phosphatase Buffer-1 (5X) |
Components | 100mM MOPS, 50mM magnesium acetate, 5mM DTT 5, 5mM EDTA, 25mM beta glycerophosphate, and 5mM sodium orthovanadate, pH 7.4 |
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Content And Storage | Ambient temperatures |
Form | Liquid |
Product Type | Kinase buffer II (5X) |
Kinase Buffer S (5X)
Kinase Quench Buffer
5X Kinase Buffer A
Shipping Condition | Approved for shipment at Room Temperature or on Wet or Dry Ice |
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Form | Liquid |
Product Type | Kinase Buffer A |
Concentration | 5X |
For Use With (Application) | Pharma & Biopharma; Protein Assays and Analysis |
Proteins and phosphoprotein may be prone to degradation following cell lysis, e.g. by endogeneous phosphatases. When running a cell-based assay, an appropriate stabilizing and blocking reagent is thus mandatory in order to prevent any remaining enzymatic activity, as well as to garantee protein integrity and quantification.
This buffer is a component of HTRF KinEASE kits developed for an optimal kinase enzymatic step. It can be ordered as a spare part.
Formulation of 5X buffer: 125 mM HEPES, pH 7.4, 0.5% Casein, 5 mg/mL Dextran-500 K, 2.5% Triton X-100, 2.5% Blocking reagent, 2.5% BSA and 0.25% Proclin-300
Stimulationreagent optimized and validatedfor use in the IP-One Gq kits.
Alt-R S.p.Cas9-GFP V3, 500ug
AlphaLISA universal buffer, 10X
New England Biolabs supplies a reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of T3, T7, Hi-T4 and Salt-T4 DNA Ligases .