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Optimized and Laser Specific
Spend less time fine tuning panels and more time discovering with BD Horizon RealBlue™ 780 reagents.
Simplicity and Support
The BD Accuri™ C6 Plus Flow Cytometer supports a variety of immunology, cell and cancer biology, plant and microbiology, and industrial applications.
Understand Single-Cell Multiomics
Unleash the power of high-dimensional biology with our unique single-cell multiomics (scM) instruments and reagents.
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Customize Your Single-Cell Experiments
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Fetal Bovine Serum Stain Buffer, BD
For the immunofluorescent staining of single-cell suspensions
BD Cytofix™ Fixation Buffer
Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time.
Fc Block , Unlabeled, Clone: 3070, BD
Monoclonal Antibody
Bovine serum albumin Stain Buffer, BD
For immunofluorescent staining of single-cell suspensions
BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit
Enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies
CD16/CD32 Rat anti-Mouse, Unlabeled, Clone: 2.4G2, BD
Rat Monoclonal Antibody
Lysing Buffer, BD
Buffered ammonium chloride-based lysing reagent
BD Perm/Wash™ Buffer
Can be used in intracellular cytokine staining to permeabilize cells and as an antibody diluent and cell wash buffer
Phosflow Perm Buffer III, BD
For the permeabilization of fixed Peripheral Blood Mononuclear Cells (PBMCs) or lysed whole blood
Brilliant Stain Buffer, BD
Designed to complement multicolor flow cytometry experiments. Brilliant Stain Buffer is a buffer for the immunofluorescent staining of cells.
BD Horizon™ Brilliant Stain Buffer
BD Horizon™ Brilliant Stain Buffer is a buffer for the immunofluorescent staining of cells.
BD GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A)
Enhance the detectability of cytokine-producing cells with immunofluorescent staining and flow cytometric analysis
Phosflow Lyse/Fix Buffer 5X, BD
For the lysing and fixing of whole blood for use with intracellular flow cytometry
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution
1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining
BD BD GolgiStop™ Protein Transport Inhibitor (containing Monensin)
Block the intracellular protein transport processes when added to in vitro- or in vivo-stimulated lymphoid cells
Featured Products
Immunoassays
BD™ Cytometric Bead Array (CBA)
Measure a variety of soluble and intracellular proteins, including cytokines, chemokines, growth factors, and phosphorylated cell signaling proteins, using flow cytometry.
Cell-Based Assays, Apoptosis
BD™ Horizon™ Fixable Viability Stain
Find a range of apoptosis detection tools that use flow cytometry, bioimaging, microscopy, and more to measure indicators at different stages across the apoptotic process.
Cell-Based Assays, Proliferation
BD Horizon Fixable Viability
Discover a series of antibodies and kits designed to detect proliferating cells.
Optimizing Experiments Using BD Horizon Brilliant Polymer Conjugates
BD Horizon Brilliant™ polymer conjugates have expanded the number of bright fluorochromes available for multicolor flow cytometry. BD Horizon Brilliant Stain Buffer, when added to samples prior to the addition of antibodies, can mitigate interactions, improving staining quality regardless of the source of reagents.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Figure 1
BD Brilliant Stain Buffer improves the performance of BV dyes in cocktails.
In the absence of BD Brilliant Stain Buffer, the CD4+ population shows non-specific interaction. Addition of BD Brilliant Stain Buffer restored the populations to their expected locations.
Figure 2
Enhanced performance of BV reagents.
In the absence of BD Brilliant Stain Buffer, these mutually exclusive populations can appear undercompensated. The addition of BD Brilliant Stain Buffer eliminates the issue, and staining returns to the expected pattern.
BD Horizon BB515 — A Brighter Alternative to FITC
BD Horizon Brilliant Blue 515 (BB515) is a dye that was exclusively developed by BD Biosciences as a brighter alternative to FITC. This dye is significantly brighter than FITC and has less spillover into the PE channel. BB515 can be detected in the same filter as FITC (e.g. 530/30).
BD Horizon BUV395 — UV-Excitable Dye with Little-to-No Spill Over
BD Horizon Brilliant Ultraviolet 395 (BUV395) is a UV-excitable dye that has been developed exclusively by BD Biosciences for instruments equipped with a 355-nm UV laser. This dye is optimal for multicolor flow cytometry because it has little-to-no spillover into other detectors. BUV395 can be detected in a 379/28 filter.
BD Horizon BUV496 — An Additional Color for the UV Laser
BD Horizon BUV496 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an Em Max at 496 nm. BUV496 has been exclusively developed by BD Biosciences for instruments equipped with a 355-nm UV laser. BUV496 can be detected in a 515/30 filter.
BD Horizon BV421 — The Brightest Dye for the Violet Laser
BV421 offers a violet-excitable dye with significantly improved brightness over other dyers offered for the violet laser. Additionally, Brilliant Violet reagents are brighter than reagents for the blue and red lasers such as PE and APC, making BV421 the brightest dye offered by BD. The brightness of the dye makes it particularly useful in multicolor applications where it can be used to better resolve dim populations. BV421 can be detected in a 450/40 filter.
BD Horizon BV510
BD Horizon BV510 provides a brighter alternative to BD Horizon V500. The improved brightness provides a brighter option for the second violet channel allowing more flexibility in panel design. BV510 is ideal for dimmer markers while BD Horizon V500 is still a good choice for bright markers. BV510 can be detected in a 525/50 filter.
BD Horizon BV605
BV605 is a tandem fluorochrome consisting of BV421 and an acceptor dye with an emission maximum of 602nm. The BV605 antibody conjugates show brightness similar to equivalent PE reagents, providing an additional bright dye choice for the violet laser. With an excitation maximum of 407nm and emission maximum of 602nm, BV605 is excited by the violet laser can can be detected with standard filter sets.
BD Horizon BV650
BD Horizon Brilliant Violet 650 (BV650) is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em Max at 650 nm. BV650 is a very bright dye, optimal for the dimmer markers in your panel.
BD Horizon BV711
BD Horizon BV711 is a tandem fluorochrome of BV421 and an acceptor dye with an Em Max at 711nm. It can be excited by the violet laser and detected in a filter used to detect Cy5.5 and Alexa Fluor 700-like dyes (such as a 710/50nm filter). Not only does BD Horizon BV711 provide an additional choice for multicolor panel design, its brightness makes it an optimal choice for dim markers.
BD Horizon BV786
BD Horizon Brilliant Violet 786 (BV786) is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 offers a bright choice for the sixth detector off the violet laser. This dye can be detected in a 780/60 filter.
BD Horizon PE-CF594 – A unique choice for blue, green and yellow-green lasers
The BD Horizon PE-CF594 dye has been developed exclusively by BD Biosciences to have improved brightness and spectral characteristics over current dyes used for the PE-Texas Red detector.
BD Cytometric Bead Array Brochure
Read how flow cytometry can be used to quantify multiple proteins simultaneously.
- BD Cytometric Bead Array Brochure (884.8 KB)
Multicolor Flow Cytometry Panels Brochure
Explore protocols, panels, and more to help further your COVID-19 research.
BD Horizon Red 718 Reagents Brochure
Learn how these reagents can help enable greater experimental insights.
- BD Horizon Red 718 Reagents Brochure (937.1 KB)
Flow Cytometry Design Journey Data Sheet
Reference this five-step guide to learn more about flow cytometry and its many applications.
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BD Horizon Brilliant Blue 515 Reagents Data Sheet
BD Horizon Brilliant Blue 515 (BB515) was developed exclusively by BD Biosciences as a brighter alternative to FITC. Compared to FITC, this dye also has less spill over into the PE channel.
BD Horizon Brilliant Dyes Brochure
BD Horizon Brilliant dyes enable resolution of cell populations that were previously obscured, opening new avenues of investigation and a deeper level of study using flow cytometry.
- BD Horizon Brilliant Dyes Brochure (3.7 MB)
BD Cytometric Bead Array Brochure
BD Cytometric Bead Array (CBA) is a flow cytometry application that allows users to quantify multiple proteins simultaneously.
- BD Cytometric Bead Array Brochure (1.5 MB)
BD Multicolor Antibody Reagents Catalog 18th Edition
Choose from our extensive portfolio of high-quality fluorescent-conjugated reagents to build your multicolor flow cytometry panels.
BD Horizon Brilliant Ultraviolet Reagents Data Sheet
BD Horizon Brilliant Ultraviolet polymer dyes are UV-excitable dyes that have been developed to expand the multicolor capabilities of flow cytometers.,
BD Horizon Brilliant Violet Reagents Data Sheet
BD Horizon Brilliant Violet unique polymer dyes enable researchers to identify cell populations with lower receptor density and resolve cell populations previously obscured.
BD Horizon PE-CF594 Reagents Data Sheet
Learn how BD Biosciences continues to expand the options for multicolor flow cytometry through the exclusive development of a dye for flow cytometers.
BD Cytometric Bead Array (CBA) Product Catalog
BD Cytometric Bead Array products are evaluated for performance characteristics including reproducibility, linearity, spike recovery and theoretical limit of detection.
BD Biosciences T Cell Research Brochure
A Solid Commitment to Research: Novel multicolor flow cytometry tools for the study of CD4+ T cell differentiation and plasticity.
BD Biosciences CD4+ T Cell Differentiation Poster
T cells play central roles in cell-mediated and humoral immunity. Several different subsets of T cells have been discovered, each with distinct functions.
- BD Biosciences CD4+ T Cell Differentiation Poster (1,003.4 KB)
BD Biosciences T Cell Reagents Product Catalog
Learn more about T Cell Reagents products, including immunology, cell biology, immunoassays, cell-based assays and other products.
BD Biosciences Stem Cell Research Reagents Product Catalog
Learn more about BD Biosciences Stem Cell Research Reagents products, including multicolor flow cytometry kits and intracellular markers.
BD Biosciences Stem Cell Research Brochure
Analysis and Isolation of Stem Cells Using Flow Cytometry. Flow cytometry is a powerful methodology for characterizing, analyzing and isolating stem cells and their derivatives.
Caspase-3 Activation: An Indicator of Apoptosis in Image-Based Assays - Application Notes
Learn about the tools for assessing cellular events: Apoptosis, Cell Cycle and Cell Proliferation.
Screening and Development of Antibodies for Use in Cell-Based Assays Application Notes
Learn about the tools for assessing cellular events and method for screening purified antibodies.
BD Biosciences NA/LE Reagents Product List
BD Biosciences offers a broad spectrum of monoclonal antibodies that possess functional activity and offer our no-azide, low-endotoxin format (NA/LE) specifically to eliminate the problems associated with azide toxicity and endotoxin effects on in vivo and in vitro functional studies.
- BD Biosciences NA/LE Reagents Product List (566.5 KB)
BD OMICS-Guard Sample Preservation Buffer Brochure
Review three datasets that showcase the versatile applications of the BD OMICS-Guard Buffer.
BD OMICS-Guard Sample Preservation Buffer Data Sheet
Access product usage, protocol, and ordering information for the BD OMICS-Guard Buffer.
BD OMICS-Guard Sample Preservation Buffer Brochure
Review three datasets that showcase the versatile applications of the BD OMICS-Guard Buffer.
BD OMICS-Guard Sample Preservation Buffer Data Sheet
Access product usage, protocol, and ordering information for the BD OMICS-Guard Buffer.
Innovations and Advancements
BD Biosciences began in the 1970s at Stanford University where Dr. Leonard Herzenberg invented fluorescence-activated cell sorter (FACS) technology. Since then, they’ve been at the forefront of single-cell analysis.
With a dedication and passion for research and development, BD Biosciences is taking a closer look at what we know about the building blocks of human life and blazing new trails in immunology.
BD Biosciences is committed to being the scientific partner of choice for your laboratory, pushing the boundaries of what’s possible at the cellular level and enabling change through informatics, genomics, proteomics, transcriptomics, and high-resolution imaging.
By setting the stage for the next decade of medical research, BD Biosciences is empowering scientists around the world to unlock the next generation of treatments and improve lives.