Test
isolated from wheat germ, dissolved in 0.05 M Tris-HCl, 0.15 M NaCl buffer, pH 8.6 and purified by affinity chromatography
Phytohemagglutinin, Phaseolus Vulgaris is crude Phaseolus vulgaris-P lectin (PHA-P) from the Red Kidney Bean.
The Phytohemagglutinin-L (PHA-L) Solution (500X) is a ready to use solution of PHA-L in aqueous buffer.
Product Type | Phytohemagglutinin-L (PHA-L) Solution |
---|---|
Product Line | eBioscience™ |
NC2606703 RIDASCREEN PEANUT
Dolichos biflorus agglutinin (DBA) binds α-linked N-acetylgalactosamine. Used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing, this lectin has also been used as a general marker of developing renal collecting ducts. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Wheat germ agglutinin (WGA) binds N-acetylglucosamine, preferentially to dimers and trimers of this sugar. It can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA also interacts with some glycoproteins via sialic acid residues (see succinylated WGA). This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Peanut agglutinin (PNA) binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and other soluble and membrane-associated glycoproteins and glycolipids. With some exceptions, the PNA receptor sequence is normally sialylated, which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid that is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Ulex europaeus agglutinin I (UAE I) binds to glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It is also an established marker for human endothelial cells. This rhodamine-labeled UAE I features a ratio of fluorophore to lectin protein that provides optimal staining (excitation 575 nm, emission 600 nm) and is supplied in solution, essentially free of unconjugated fluorophore.
WHEAT GERM AGGLUTININ (WGA) SEPAROPORE 4B-MB: Wheat Germ Agglutinin (Lectin) Separopore 4B Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group ...
WHEAT GERM AGGLUTININ (WGA) SEPAROPORE 4B-MB: Wheat Germ Agglutinin (Lectin) Separopore 4B Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group ...
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 dye has excitation/emission at 593/614 nm.
This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue fluorescent CF350 dye has excitation/emission at 347/448 nm.