Test
Regulates packaging in transcriptional level. Works for both lenti-and retro-viral packaging. Increases viral particle production up to 10-fold. Achieve high titer at low cost. Novel cocktail of small molecules. Easy to use.
Lenti-X Packaging Single Shots (VSV-G) provide an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because Lenti-X Packaging Single Shots (VSV-G) consist of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. No. 632180), in a 10 cm dish. 16 Rxns
This retrovirus titer boost is a novel recipe of small molecules designed for increasing / boosting retrovirus packaging efficiency. Retrovirus 10X Titer-Up stably regulates the viral RNA packaging on the transcriptional level, which can enhance production of retroviral particles for up to 10 fold.
Quantitatively precipitate any retrovirus with Retro-Concentin™. Instead of using lengthy spins in an ultracentrifuge, Retro-Concentin enables concentration of retroviruses directly from culture medium with a simple one (or an optional two) low-speed centrifugation steps. In addition, Retro-Concentin stabilizes the retroviruses for frozen storage, providing an additional advantage over other concentration methods. Each preparation can handle up to 200 mL of retroviral supernatant, which is compatible with most centrifuges, and the resulting pellet can be dissolved in the volume of buffer that meets your experimental requirements.
NC2861687 RETROVIRAL PACKAGING MIX 200 U
NC1628722 VIRUS PROTECTION MEDIUM
Clone your gene of interest into Cell Biolabs' pBABE-puro Retroviral Vector and then package into a retroviral particle using a retroviral packaging cell or co-transfect with retroviral packaging constructs into 293T cells.
BD Retro-X Universal Packaging System, 8 RXN, Use w/any Retroviral Vector, 100% Cell Transduce, Efficiently Transduce Hard-To-Transfect Cell Lines, Easily Create Stable Cell Lines, Preparation and Storage: -196degreeC
pCMV-VSV-G Envelope Vector can be used in co-transfections with other packaging plasmids to make recombinant lentivirus or retrovirus.