Invitrogen™ Guanosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt (BODIPY™ FL GTP-γ-S, Thioester)

Fluorescence may be recovered on binding to G-proteins

Supplier:  Invitrogen™ G22183

Catalog No. G22183

Description

BODIPY FL GTP-γ-S binds to GTP-binding proteins. To create this fluorescent nucleotide, the BODIPY FL fluorophore was linked through the γ-thiol of GTP-γ-S. The fluorescence of BODIPY FL GTP-γ-S is quenched approx. 90% relative to that of the free dye, but may be recovered on binding to G-proteins. In addition to its potential use for binding studies, the BODIPY FL GTP-γ-S thioether is an important new substrate for Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins.

Drug Discovery and Development, Enzyme and Protein Activity Assays, Phosphatase Activity, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

Order Info

Shipping Condition: Wet ice

Specifications

• Product Type: Guanosine 5'-O
• Labeling Method: Chemical Labeling, Direct Labeling
• Concentration: 5 mM
• Quantity: 50 μL

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Shipping Condition: Wet Ice
• Label or Dye: BODIPY™ FL
• Product Line: BODIPY™

For Research Use Only. Not for use in diagnostic procedures.