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Thermo Scientific™ Pierce™ Rapid Antibody Isotyping Kit - Mouse
Identify mouse monoclonal antibody class and subclass in 5 minutes with these single-use, cassette-based lateral-flow assays.
Supplier: Thermo Scientific™ 26178
Description
Thermo Scientific™ Pierce Rapid Mouse Antibody Isotyping Kits are single-use, cassette-based kits for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.
The kits are composed of small cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. The kits determine mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM.The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.
Highlights:
Long shelf life – stable for more than 1 year at room temperature
Single-step – add diluted antibody sample to the loading-well of cassette
Fast – within 5 minutes a band appears that indicates the antibody isotype
Sensitive – detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10ng/mL
Specific – assay does not crossreact with fetal bovine serum (FBS)
Reliable – results are similar to the standard ELISA-based isotyping assays
Includes:
Ten pouches, each containing two (Part No. 26178) or three (Part No. 26179) cassettes, plus a bottle of sample diluent
Specifications
| 10 Tests | |
| Pierce™ | |
| Target-Specific | |
| Sufficient For: 10 isotype determinations • Isotyping Cassettes (two per pouch), 10 pouches • Sample Diluent, 45 mL Store kit at 2–8°C. |
| Membrane Blot | |
| Isotyping Assay | |
| Chromogenic | |
| Pierce Rapid Isotyping Kit - Mouse |
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