NGF Recombinant Rabbit Monoclonal Antibody (SI79-01), Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Supplier:  Thermo Scientific MA532067

Catalog No. PIMA532067

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

Specifications

• Antigen: NGF
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide, pH 7.4
• Gene Accession No.: P01138, P01139, P25427
• Gene Symbols: NGF
• Immunogen: Synthetic peptide within Human NGF aa 192-241
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Zebrafish
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot, Western Blot
• Clone: SI79-01
• Conjugate: Unconjugated
• Gene: NGF
• Gene Alias: B nerve growth factor, beta ngf, beta-nerve growth factor, BetaNGF, beta-NGF, bNGF, b-NGF, HSAN5, MGC161426, MGC161428, nerve growth factor, nerve growth factor (beta polypeptide), nerve growth factor, beta, nerve growth factor, beta polypeptide, nerve growth factor, beta subunit, NGF, NGF b, NGF β, Ngfb, NGFbeta, NGFβ, &beta, nerve growth factor, &beta, NGF, βNGF
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 18049, 310738, 4803
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid