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Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit

The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.

Supplier:  Thermo Scientific™ K2561

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Catalog No. FERK2561


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Includes: First Strand Enzyme Mix, 4X First Strand Reaction Mix, Second Strand Enzyme Mix, 5X Second Strand Reaction Mix, 0.5M EDTA ( pH 8.0), RNase I (10 u/μL), Control RNA (0.5μg/μL), Oligo(dT)18 Primer, 100μM, 0.5μg/μL, Random Hexamer Primer, 100μM, 0.2μg/μL,Water, nuclease-free, Detailed Protocol

Description

Description

Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains pre-mixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Maxima H Minus Double-Stranded cDNA Synthesis Kit include:

  • Efficient synthesis of full-length double-stranded cDNA
  • Fast—procedure completed in less than two hours
  • Convenient—pre-mixed components
  • Complete—includes all primers, controls and residual RNA removal reagents

Applications

  • Full-length double-stranded blunt-end cDNA synthesis for cloning
  • Double-stranded cDNA library construction

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

10 reactions
Maxima
10 Reactions
Cloning
10 Reactions
RNA
50°-55° C
cDNA Synthesis Kit
Maxima H Minus
Double Stranded cDNA
Separate Components
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For Research Use Only. Not for use in diagnostic procedures.