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MilliporeSigma™ Immobilon Signal Enhancer for Immunodetection

MilliporeSigma™ Immobilon™ Signal Enhancer for Immunodetection combines signal amplification and blocking in one ready-to-use reagent. Amplifies low signal intensity Western blots without amplifying the noise

Supplier:  MilliporeSigma™ WBSH0500

Catalog No. WBSH0500

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Includes: Immobilon Signal Enhancer for Immunodetection 500ML



  • Immobilon™ Signal Enhancer is designed for use in Western blotting applications when low signal intensity is observed. It combines signal amplification and blocking in one ready-to-use reagent.
  • Enhancement of the antibody-antigen reaction can produce a 2 to 10-fold increase in signal intensity over traditionally used reagents. The reagent is formulated for use in both membrane blocking and antibody dilution steps and can be used for traditional immunodetection or with the SNAP i.d.™ Protein Detection System.
  • Save Primary Antibody: When the signal is strong, use Immobilon™ Signal Enhancer to reduce the amount of valuable primary antibody required
  • Immobilon™ Signal Enhancer is compatible with PVDF as well as nitrocellulose blotting membranes and can be used with chromogenic, chemiluminescent, and fluorescent detection methods.
  • In applications where sufficient signal is observed, but the primary antibody availability is limited, Immobilon™ Signal Enhancer can be used to reduce the amount of valuable primary antibody required





Western Blotting, immunodetection
500 mL




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