GFP Monoclonal Antibody (GF28R), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA515256

Catalog No. PIMA515256

Description

MA5-15256 recognizes native and denatured forms of GFP (Green Fluorescent Protein) and its many popular variants: EGFP, YFP, EYFP, and CFP. MA5-15256 has successfully been used in immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications. MA5-15256 may be stored at 4oC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20oC, avoiding freeze/thaw cycles.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
• Clone: GF28R
• Conjugate: Unconjugated
• Gene Alias: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
• Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Tag

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov