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Thermo Scientific™ Evolution™ One/One Plus UV-Vis Spectrophotometers

Thermo Scientific™ Evolution™ UV-Vis Spectrophotometers combine reliable and versatile hardware with easy-to-learn, easy-to-use Thermo Scientific™ Insight™ Pro Software. Together they will meet the diverse needs your organization requires from an analytical tool.

Supplier:  Thermo Scientific™ 840341400

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Catalog No. 14-385-500

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For routine, complex research and advanced applications, including current compliance, the Thermo Scientific™ Evolution™ One/One Plus UV-Vis Spectrophotometer series brings together reliable, versatile hardware, a broad range of accessories, with easy-to-use, easy-to-learn Thermo Scientific™ Insight™ Pro Software.

For routine to research analysis, this instrument series offers real innovations that provide enhanced usability and performance - without added complexity. With straightforward software, cutting-edge instrumentation and a wide selection of accessories, the Evolution One and One Plus UV-Vis Spectrophometers work with you to perform experiments the way you want - for the results you need.

Optimized Engineering Delivers Ultimate Performance:
  • Double-beam geometry with fixed 1nm or variable spectral bandwidth options
  • Application Focused Beam Geometry (AFBG) technology enables the best performance from microcells, solid samples, and fiber optic probes (Evolution One Plus Only)
  • Xenon flash lamp requires no warm-up time allowing instant measurements
  • Meet chemical, materials science, or biological sampling and measurement needs with a broad range of accessories
Insight Pro Software—Easy-to-learn, easy-to-use
  • Reduce training time: Optimized user interface with prompting software defines clear step-by-step workflow and is easy to teach and learn
  • Prevent costly sampling and measurement errors: Create dedicated workflows for complex assay and transform multi-step workflows into one simplified, easy-to-follow method suitable for any level of user with Customized User Environment (CUE)
  • Always current: Whether it is user inputs or application requirements, or new regulations, Insight Pro Software is continuously improving with quick upgrades to deliver greater capabilities and efficiencies for your lab’s needs
Accessories for all your Sampling Needs:
  • Wide accessory selection with various temperature control options, sample changers, sippers and solid sampling devices complete your analysis
  • Choice of Peltier Single Cell Holder or a Smart 8-Cell Peltier system for temperature control and sample monitoring from 0° to 100 °C
  • Easy software integration gives you complete control of your measurements

Regulatory/Quality Control Environments:
To use the EvolutionOne/One Plus UV-Vis Spectrophotometers in a regulated or quality control environment, we offer a wide selection of compliance products and services to reduce implementation time and assure system performance.

Evolution One/One Plus Security and Validator Bundles include:
  • Evolution One or One Plus UV-Vis Spectrometer
  • INSIGHT Pro with Security Software
  • Validator for Insight Pro US Ltr or INTL A4 Paper Version
  • Qualification Filter Kit for UV-Vis
Evolution One/One Plus USP/EP Standards Pharma Bundles include:
  • Evolution One or One Plus Spectrometer
  • INSIGHT Pro with Security Software
  • Validator for Insight Pro US Ltr or INTL A4 Paper Version
  • USP and EP UV Standards Set
  • USP and EP Visible Standards Set


±0.001 A, 200–800 nm, 1.0 nm SBW, smoothed
Dual Silicon Photodiodes
Xenon Flash
1 nm
190 to 1100 nm
100/240 V, 50/60 Hz selected automatically, 150 W maximum
0A: ≤0.00015 A
1A: ≤0.00025 A
2A: ≤0.00050 A
260 nm, 1.0 nm SBW, RMS<
Photometric Accuracy (60mg/L K2Cr2O7): ±0.010A
Stray Light: ≤1%T at 198 nm: KCI; ≤0.05%AT at 220 nm: Nal, Kl
Wavelength Accuracy: ±.5 nm 541.9, 546.1nm Hg emission lines, ±0.8 nm full range
Wavelength Repeatability: ≤0.05 nm, repetitive scanning of 546.1 nm Hg emission line
-0.3 to 4.0 A
1 A: ±0.0002 A
<1 to 6000 nm/min. (Variable)
150 W max.
10, 5, 2, 1.0, 0.5, 0.2, 0.1 nm
32 lb.
Evolution One UV-Vis Spectrophotometer
593 x 475 x 266 mm (23.3 x 18.7 x 10.6 in.)
Evolution One
UV-Vis Spectrophotometer
<0.0005 A/hr., 500 nm, 1.0 nm SBW, 1 hour warmup
>5 years or longer if not using live signal
Double-beam with sample and reference cuvette positions Czerny-Turner Monochromator
1 A: ±0.004 A, 2 A: ±0.008 A, Measured at 440 nm using neutral density filters traceable to NIST
>3.5 A
Absorbance, % Transmittance, % Reflectance, Kubelka-Munk, log (1/R), log (Abs), Abs*Factor, Intensity
Instrument (2 years), Lamp (3 years)
±0.5 nm (541.9, 546.1 nm mercury lines), ±0.8 nm (full range 190 to 1100 nm)
≤0.05 nm (546.1 nm mercury line, SD of 10 measurements)
14.5 kg
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