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Corning™ Fetal Bovine Serum, Premium (Tetracycline Negative)

Fetal Bovine Serum often contains the antibiotic tetracycline (tet) and researchers using very sensitive tet-inducible gene expression systems encounter problems with unintended expression as a result. Serum containing minimal levels of tetracycline is needed to ensure accurate gene expression.

Supplier:  Corning™ 35075CV

Catalog No. MT35075CV


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Description

Description

Fetal Bovine Serum (FBS) is a time tested, universal supplement used in cell culture. It provides many compounds that have been shown to satisfy specific metabolic requirements for the culture of cells, including hormones, growth factors, cell attachment factors, pH buffer, protease inhibitors, vitamins, minerals, lipids, and a variety of undefined components. Serum also protects against toxic effects associated with pH change, the presence of heavy metal ions, endotoxin, and proteolytic activity.

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Sera Processing

Using industry leading techniques, blood is aseptically collected at abattoirs, centrifuged to isolate the serum and immediately frozen for testing and further processing. The raw sera is then true pooled and is triple 0.1 micron sterile filtered with a Sterility Assurance Level (SAL) of 10-3. Every lot then must pass USP <71> and EP 2.6.1 sterility testing, growth promotion testing, as well as test negative for mycoplasma and number of industry-monitored viruses. Authenticity of the serum is verified using industry leading measurements of Gamma-Glutamyl Transferase and Corning’s high internal standard of ≤ 10 IU/L. Additional testing includes endotoxin, hemoglobin, pH, osmolality, total protein, and a biochemical profile that contains various metals, proteins, hormones and other metabolites.

EDQM Certificates of Suitability

Fetal Bovine Serum, United States Origin

This product has been granted a Certificate of Suitability, R0-CEP 2018-271, by the European Directorate for the Quality of Medicines (EDQM) certifying that the product meets the criteria described in the current version of the monograph no. 1483 of the European Pharmacopoeia “Product with risk of transmitting agent of animal spongiform encephalopathies.”

Origin Statement

Corning’s premium FBS is only collected from government approved abattoirs in the United States, Australia, and New Zealand. All fetal bovine serum is derived from fetuses collected from healthy bovine cows and heifers that have passed ante and post mortem inspection and were found free of contagious diseases.

Corning’s regular FBS is derived from government registered facilities in North, Central, and South America and outside the United States. All fetal bovine serum is derived from fetuses collected from healthy bovine cows and heifers that have passed ante and post mortem inspection and were found free of contagious diseases. The material was exported from the country of origin accompanied with a Veterinarian Health Certificate. 

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Order Info

Shipped in frozen form.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

cGMP
-25° to -15°C
500 mL
Triple-filtered, 0.1 μm
<0.05EU/ mL
Test and Report
Test and Report
Amber Yellow to Reddish
Fetal Bovine Serum
Sterile
Bovine Fetus
Tetracycline Negative FBS is for cell culture applications using tet-inducible gene expression systems and any other application in which the presence of tetracycline would prove disruptive.
Test and Report
USDA Inspected
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Research use only.