Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO

Use this 20K MWCO semi-permeable membrane to remove salts and contaminants with maximal sample recovery.

Catalog Number	Mfr. No.	Sample Volume (Metric)
PI66005	Thermo Scientific™ 66005	0.5 mL
PI66003	Thermo Scientific™ 66003	3 mL
PI66012	Thermo Scientific™ 66012	12 mL
PI66030	Thermo Scientific™ 66030	30 mL

Description

Slide-A-Lyzer Dialysis Cassettes (20K MWCO) facilitate the simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules larger than 20,000 Daltons. They are available in four sizes to accommodate sample volumes between 0.5 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing faster buffer exchange, and more complete sample recovery.

Slide-A-Lyzer Dialysis Cassettes (20K MWCO) facilitate the simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules larger than 20,000 Daltons. They are available in four sizes to accommodate sample volumes between 0.5 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing faster buffer exchange and more complete sample recovery. Slide-A-Lyzer cassettes can be used for a wide range of applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips, or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume
• Color-coded—purple cassette frame specifies cassettes with the 20,000 MW pore size membrane

Membrane specifications:
• Composition: regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2 mil (22.5 to 30 μm)
• Hydration time required before use: 2 minutes
• Glycerol content: none
• Sulfur content: 0.04%
• Heavy metals content: trace

Volume ranges:
• 0.5 mL = 0.1 to 0.5 mL
• 3 mL = 0.5 to 3 mL
• 12 mL = 3 to 12 mL
• 30 mL = 12 to 30 mL

Specifications

• Content And Storage: Store at room temperature
• Membrane: Cellulose Membrane, Dialysis
• MWCO: 20 kDa

• Purification Target: Buffer Exchange, Protein
• Format: Cassette
• Product Line: Slide-A-Lyzer™