Thermo Scientific™ 96 Well Plate, UpCell Temperature Responsive Surface, Pack of 1

Color:Clear; Sterility:Sterile; Lid:With Lid

Supplier:  Thermo Scientific™ 174897

Catalog No. 03150018

Description

Support non-enzymatic harvesting of adherent cells for preservation of cell viability and surface proteins with the temperature-responsive surface of Thermo Scientific™ Nunc™ UpCell™ Microplates. Simply transfer the culture to room temperature to collect cells in suspension.

Thermo Scientific Nunc UpCell Surface
UpCell surface is a temperature-responsive cell culture surface that enables non-enzymatic harvesting of adherent cells for preservation of cell viability and surface proteins.
A temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) polymer is grafted onto the standard cell culture treated polystyrene surface. At 37˚C, the covalently bound polymer allows cells to attach to the dish and grow as normal. At room temperature, the PNIPAAm binds water and swells, releasing the attached cells together with the extracellular matrices. The cells are dissociated from the culture substrate without trypsin.

Features:

• The hydrophicility of the UpCell surface changes with the external temperature, allowing cells to detach
• No need for dissociation enzymes, ensuring intact cell surface proteins
• No need for physical scraping, resulting in high cell viability
• Shelf Life: 3 years from date of manufacturing

Recommended for:

• Culture passaging
• Subsequent single-cell analyses (e.g. flow cytometry)
• For research use only

Specifications

• Description: 96F well plate, UpCell, clear, with lid, Sterile (Pack of 1, Case of 8)
• Volume (Metric) Working: 200 μL
• Quantity: 8/Cs.
• No. per Case: 8
• Surface Treatment: UpCell-Treated, Temperature Responsive
• No. of Wells: 96
• Array: 8 x 12
• Packaging: 1/Pk., 8/Cs.
• Well Shape: Flat

• Material: Polystyrene
• Type: Microplate
• No. per Pack: 1
• Color: Clear
• Sterility: Sterile
• Certifications/Compliance: All microplates manufactured by Nunc meet the recommendation of American National Standards (ANSI) (ANSI/SBS 1-2004)
• Product Line: Nunc™
• Barcode: Custom Barcoding Available