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Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
$66.50 - $275.00
Specifications
| Product Type | Stand-alone enzyme |
|---|---|
| For Use With (Application) | LAMP, RT-LAMP |
| Polymerase | Lyo-ready Bst DNA Polymerase |
| Optimal Reaction Temperature | 65°C |
| Shipping Condition | Dry Ice |
| Catalog Number | Mfr. No. | Concentration | Quantity | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Concentration | Quantity | Price | Quantity & Availability | ||||
A56655
|
Invitrogen™
A56655 |
6 U/μL | 1200 U |
Each for $66.50
|
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A56656
|
Invitrogen™
A56656 |
6 U/μL | 6000 U |
Each for $275.00
|
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A56657
|
Invitrogen™
A56657 |
40 U/μL | 1200 U |
Each for $66.50
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Description
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.
Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs
Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)
Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.
Specifications
| Stand-alone enzyme | |
| Lyo-ready Bst DNA Polymerase | |
| Dry Ice |
| LAMP, RT-LAMP | |
| 65°C | |
| ≤10 minutes |
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