Invitrogen™ iBind™ Western Starter Kit

Complete kit for automated Western processing

Supplier:  Invitrogen™ SLF1000S

Catalog No. SLF1000S

Includes: iBind Western Device, 10 iBind Cards, iBind Solution Kit

Description

The iBind Western Starter Kit includes an iBind Western Device as well as all of the consumables and reagents necessary to get started using this automated Western-processing device that performs every step in the Western protocol from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source. Each solution is released from the iBind device wells to an iBind Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

• Simple, automated solution
• No external power source
• Superior reproducibility over traditional manual methods
• Better antigen antibody interaction—solutions flow between the iBind Card and membrane for maximum interaction between protein and antibody
• Minimizes hands-on time
• Highly reproducible and compatible with all Western protocols
• Set up time is fast—ready for the detection step within 2.5 hr.
• Carry out detection right away or wait until the next day without affecting performance
• Works exclusively with iBind Cards and the iBind Solution Kit to process one mini-membrane for immunodetection
• Provides automated convenience and superior reproducibility over traditional manual methods

Kit components may also be purchase separately.

Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

• Membrane Compatibility: Nitrocellulose, PVDF
• Product Line: iBind™
• Product Type: Starter Kit
• Content And Storage: • 1 iBind™ Western Device
  • 10 iBind™ Cards (store at room temperature)
  • 1 iBind™ Solution Kit (store at 2–8°C)

• Detection Method: Chromogenic, Chemiluminescent, Fluorescent
• Run Time: ∼2.5 hours
• Capacity: 1 mini blot (8 x 8 cm)

For Research Use Only. Not for use in diagnostic procedures.