Invitrogen™ DNase I, Amplification Grade

Purified and tested for nondetectable levels of RNase contamination

Supplier:  Invitrogen™ 18068015

Catalog No. 18-068-015

Includes: DNase I, Amp Grade (100U), 10X DNase I Reaction Buffer, 25mM EDTA (pH 8.0)

Description

DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder.

• Specific activity is >10,000 units/mg
• Source: Purified from bovine pancreas
• Performance and quality testing: Ribonuclease assay with RNA ladder and ability to digest single-stranded and double-stranded DNA to oligonucleotides are determined
• Unit definition: One unit increases absorbance of high molecular weight DNA solution at rate of 0.001 A₂₆₀ units/min./mL of reaction mixture at 25°C
• Unit reaction conditions: 0.1M sodium acetate (pH 5.0), 5mM MgCl₂, 50 μg/mL calf thymus DNA, and enzyme in 1mL for 10 min. at 25°C

Removing DNA from RNA and protein preparations, PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription

Order Info

Shipping Conditions: Approved for shipment on wet or dry ice

Specifications

• Content And Storage: Contents:
  1 vial of DNase I, Amp Grade (100 U)
  1 vial of 10X DNase I Reaction Buffer (1000 μL)
  1 vial of 25 mM EDTA (pH 8.0) (200 μL)
  
  Store at -20°C in a non-frost-free freezer.
• Enzyme: DNase
• Quantity: 100 U

• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Compatible Buffer: Reaction Buffer

For Research Use Only. Not for use in diagnostic procedures.