Invitrogen™ Biotin-14-dCTP

Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling

Supplier:  Invitrogen™ 19518018

Catalog No. 19-518-018

Includes: Biotin-14-dCTP is supplied at 0.4mM in 100mM Tris-HCl (pH 7.5), 0.1mM EDTA.

Description

About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N<sup>4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50μg of DNA.

Performance and quality testing
Purity is evaluated by reverse-phase HPLC.</sup>

• Convenience and safety: probe preparation generates no radioactive waste
• Stability: probes remain active longer than isotopically labeled probes
• Versatility: biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding

DNA and RNA Purification and Analysis, DNA Labeling, Nucleic Acid Labeling and Oligo Synthesis

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Labeled Nucleotide
• Content And Storage: Biotin-14-dCTP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.
• Labeling Method: Direct Labeling
• Quantity: 50 nmol

• Concentration: 0.4 mM
• Label or Dye: Biotin & Other Hapten
• Shipping Condition: Dry Ice
• Form: Liquid

For Research Use Only. Not for use in diagnostic procedures.