Invitrogen™ BioPrime™ Array CGH Genomic Labeling System

A high-performance labeling kit that enable reproducible labeling of genomic DNA samples for array-based Comparative Genomic Hybridization (CGH)

Supplier:  Invitrogen™ 18095011

Catalog No. 18-095-011

Includes: exo-Klenow fragment (40 U/μL), 2.5X random primer solution (octamers), control DNA (Salmon Sperm), stop buffer, and distilled water. The BioPrime™ Plus Systems and Modules contain the Alexa Fluor™ modified dNTP mixes or amine modified dNTP mixes and the Alexa Fluor™ NHS Esters. The standard BioPrime™ and BioPrime™ Plus Array CGH Genomic Labeling Systems also contain purification columns and purification buffers.

Description

Using the BioPrime Plus Array CGH Genomic Labeling System, you can expect:
––High yields of fluorescently labeled genomic DNA
–signal-to-noise ratios
–detection of gene copy number variations.


These systems combine a highly concentrated exo-Klenow fragment of DNA polymerase I and random primers to effectively label genomic targets for sensitive genomic profiling experiments. Exo-Klenow is a mutant of the large fragment of the DNA polymerase I holoenzyme that has both 5' - 3' and 3' - 5' exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, enabling robust yields and efficient incorporation of Alexa Fluor™ AHA fluorescent nucleotides. In the BioPrime Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor AHA modified nucleotides (direct labeling) or amino-allyl modified nucleotides (indirect labeling) are incorporated as the polymerase extends from the priming sites. The labeled samples are then purified to remove contaminants prior to denaturing and hybridization to a microarray (direct labeling systems) or coupled to the Alexa Fluor NHS ester (indirect labeling systems) prior to hybridization. Available in a direct labeling format.

Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research

Order Info

Shipping Condition: Labeling Module, Dry Ice; Purification Module, Room Temperature

Specifications

• Product Type: Genomic Labeling System
• Content And Storage: Table 1 summarizes the components of the various BioPrime™ Array CGH Labeling Systems. The BioPrime™ Core Module is found in all standard BioPrime™ and BioPrime™ Plus Systems and Modules. It contains exo-Klenow fragment (40 U/ μl), 2.5X random primer solution (octamers), control DNA (Salmon Sperm), stop buffer, and distilled water. Store at -20°C. The BioPrime™ Plus Systems and Modules contain the Alexa Fluor™ modified dNTP mixes or amine modified dNTP mixes and the Alexa Fluor™ NHS Esters. Store at -20°C. The standard BioPrime™ and BioPrime™ Plus Array CGH Genomic Labeling Systems also contain purification columns and purification buffers. Store at room temperature. All components are guaranteed for 6 months when properly stored. See Table 1 for product component summary.
• Label or Dye: Cy™3, Cy™5, Alexa Fluor™-Labeled Nucleotides
• Labeling Target: Genomic DNA
• Product Line: BioPrime™
• Quantity: 30 Reactions

• Detection Method: Fluorescent
• Final Product Type: Probes (Labeled DNA)
• Includes Label or Dye: No
• Labeling Method: Direct Labeling
• For Use With (Application): Chromatin Biology

For Research Use Only. Not for use in diagnostic procedures.