Invitrogen™ 5' RACE System for Rapid Amplification of cDNA Ends, version 2.0

Suitable for rapid amplification of cDNA ends (RACE) between defined point in mRNA and the 5' end

Supplier:  Invitrogen™ 18374058

Catalog No. 18-374-058

Includes: The 5' RACE System is supplied with the components listed in Table 1.

Description

The 5' RACE System provides a set of prequalified reagents intended for synthesis of first-strand cDNA, purification of first-strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR. Control RNA, DNA, and primers are provided for monitoring system performance. The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5' end information of mRNA.

• Uses advanced capabilities of SuperScriptII RNase H- RT for greater first-strand cDNA yields and increased cDNA lengths
• Prepares specific cDNA from up to 1μg of total or poly(A)+ RNA in one reaction
• Offers improved first-strand cDNA purification procedure that yields cDNA suitable for tailing
• Uses recombinant TdT with optimized reaction conditions in PCR-compatible buffer to provide efficient primer annealing site for PCR

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

Specifications

• Product Type: cDNA library
• Content And Storage: The 5´ RACE System is supplied with the components listed in Table 1. Store the reagents at -20°C and the DNA purification components at +4°C.
• For Use With (Application): cDNA Libraries & Library Construction
• Quantity: 10 Reactions

For Research Use Only. Not for use in diagnostic procedures.