Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Catalog Number	Mfr. No.	Quantity
10-569-010	Gibco™ 10569010	500 mL
10-569-044	Gibco™ 10569044	10 x 500 mL
10-569-069	Gibco™ 10569069	5 L
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10-569-077	Gibco™ 10569077	10 L
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Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, Sodium Pyruvate, GlutaMAX™, Phenol Red
Without: HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Classification: Animal Origin-free
• Form: Liquid
• With Additives: High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

• Concentration: 1 X
• Sterility: Sterile-filtered
• Without Additives: No HEPES

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.