Thermo Scientific™ GeneJET Genomic DNA Purification Kit

Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.

Supplier:  Thermo Scientific™ K0722

Catalog No. FERK0722

Description

Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.

The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.

Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources
• Efficient—high yields of high molecular weight genomic DNA
• Fast—20 minute procedure following cell lysis
• Convenient—spin columns are capped and assembled with collection tubes

Applications
Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting

Specifications

• Column Type: Spin Column
• Isolation Technology: Silica Spin Column
• Scale: Mini
• Final Product Type: gDNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Yield: ≤30 μg

• Content And Storage: • 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening
  • 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening
  • 55 mL Digestion Solution; room temperature
  • 2 x 60 mL Lysis Solution; room temperature
  • 40 mL Wash Buffer I (concentrated); room temperature
  • 40 mL Wash Buffer II (concentrated); room temperature
  • 150 mL Elution Buffer; room temperature
  • 250 GeneJET Purification Columns with Collection Tubes; room temperature
  • 250 Collection Tubes; room temperature
  
  
• Sample Type: Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
• Elution Volume: 200 μL
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Quantity: 250 Preps
• Test Time: 20 min. (After sample homogenization)
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Cells: ≤10⁶
  Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)
  Tissue: ≤20 mg
  Whole Blood: ≤200 μL
  Yeast: ≤10⁸ cells

For Research Use Only. Not for use in diagnostic procedures.