Invitrogen™ Precision gRNA Synthesis Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

Supplier:  Invitrogen™ A29377

Catalog No. A29377

Includes:

BOX 1

• 5X TranscriptAidTM Reaction Buffer
• DNase I, RNase-free (1 U/μL)
• Nuclease-free water
• NTP mix (100 mM each of ATP, GTP, CTP,
• UTP in Tris buffer)
• Control gRNA forward and reverse primers (10 μM mix) 2

BOX 2

• Binding Buffer
• Wash Buffer 1 (concentrated)3
• Wash Buffer 2 (concentrated)4
• Nuclease-free water
• Gene JETTM RNA Purification Micro Column & Collection Tubes
• Elution Tubes, 1.5 mL

Description

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short ‘one-pot’ PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.

Features of the GeneArt Precision gRNA Synthesis Kit include:

• Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
• High yield (>10 ug) and concentration (>200 ng/uL) of gRNA

For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Recommended Storage

gRNA Prep Kit, store at -5 to -30°C and gRNA Cleanup Kit, store at room temperature

Specifications

• Content And Storage: • gRNA Prep Kit, store at -5° to -30°C
  • gRNA Cleanup Kit, store at room temperature
• Format: Kit
• Technique: CRISPR-Cas9
• Promoter: T7
• Sufficient For: 25 Reactions
• Concentration: 200 ng/μL
• No. of Reactions: 25 Reactions

• Form: Liquid
• Reaction Speed: Fast
• Product Type: gRNA Synthesis Kit
• Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
• Yield: >10 μg at >200 ng/μL of gRNA
• Final Product Type: RNA
• Starting Material: Oligos (DNA)

For Research Use Only. Not for use in diagnostic procedures.