Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Supplier:  Invitrogen™ A21178

Catalog No. A21178

Includes:

• CRISPR OFP Nuclease Vector, linearized
• 10X Oligonucleotide Annealing Buffer
• DNase/RNase-free Water
• 5X Ligation Buffer
• T4 DNA Ligase
• U6 Forward Sequencing Primer
• Control, double stranded oligonucleotide
• OneShot(TM) TOP10 Chemically Competent Cells

Description

Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter.

GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells.

The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. Competent cells included for added convenience and highest cloning efficiencies.

GeneArt™ CRISPR Nuclease Vector system offers:

• Easy-to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard to transfect or difficult to modify cell

For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Compliance

CoA

Recommended Storage

Store in freezer (-5 to -30°C)

Specifications

• Cloning Method: Restriction Enzyme/MCS
• Detection Method: Primer-probe
• Format: Kit
• Technique: CRISPR-Cas9
• Product Type: Crispr Nuclease Vector With ofP Reporter Kit
• Protein Tag: OFP fusion
• Sufficient For: 10 Reactions
• No. of Reactions: 10 Reactions
• Starting Material: Oligos (DNA)

• Content And Storage: • CRISPR OFP Nuclease Vector, linearized
  • 10X Oligonucleotide Annealing Buffer
  • DNase/RNase-free Water
  • 5X Ligation Buffer
  • T4 DNA Ligase
  • U6 Forward Sequencing Primer
  • Control, double stranded oligonucleotide
  Store the above at -5 to -30°C.
  
  • OneShot™ TOP10 Chemically Competent Cells
  Store at -80°C.
• Form: Liquid
• Reaction Speed: Fast
• Product Line: GeneArt™
• Promoter: U6, CMV
• Reporter Gene: OFP
• For Use With (Application): Gene Expression, Cloning
• Overhang: 3' overhangs

For Research Use Only. Not for use in diagnostic procedures.