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Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
$126.60 - $317.65
Specifications
| Concentration | 1 U/μL |
|---|---|
| Enzyme | Phosphatase |
| Compatible Buffer | PCR Buffer, Enzyme Buffer |
| Product Type | Alkaline Phosphatase |
Description
FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.
FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 minutes at 37°C. The enzyme is inactivated in 5 minutes at 75°C . Therefore, removal of alkaline phosphatase is not required prior to ligation.
Highlights
- Recombinant enzyme
- Fast dephosphorylation -10 minutes at 37°C
- Fast and complete inactivation -5 minutes at 75°C
- Simultaneous digestion and dephosphorylation of vector DNA
- 100% active in restriction enzyme and PCR buffers
- One protocol for all types of DNA ends:
- 5'-overhangs
- 3'-overhangs
- blunt-ends
- single nucleotides
- PCR clean-up in conjunction with Exo I
- Protein dephosphorylation
Specifications
| 1 U/μL | |
| Phosphatase | |
| PCR Buffer, Enzyme Buffer | |
| Alkaline Phosphatase |
For Research Use Only. Not for use in diagnostic procedures.
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