MilliporeSigma™ Osteopontin, HisTag™, Mouse, Recombinant Protein, Mouse

Supplier:  MilliporeSigma™ 49926050UG

Catalog No. 49-926-050UG

Description

Recombinant, mouse osteopontin fused at the C-terminus to a His·Tag™ sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴ (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca²⁺-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.

Specifications

• Formulation: Lyophilized from 0.2μM sterile-filtered PBS, 50μg BSA/μg rmOPN.
• Purification Method: SDS-PAGE
• Immunogen: Osteopontin
• Regulatory Status: RUO
• Species: Mouse
• Form: Lyophilized

• Preparation Method: Reconstitute to ≥100μg/mL with sterile PBS.
• Quantity: 50μg
• Storage Requirements: Following reconstitution, aliquot and freeze (−70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at −70°C. Avoid freeze/thaw cycles of stock solutions.
• Conjugate: His-Tag
• Recombinant: Recombinant
• Purity or Quality Grade: ≥95%