DYKDDDDK Tag Monoclonal Antibody (FG4R), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA191878

Catalog No. MA191878

Description

This antibody detects Flag tag from various samples. similar epitope as the FLAG tag from Sigma MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications. The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH. MA1-91878 may be stored at 4oC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20oC, avoiding freeze/thaw cycles.

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

Specifications

• Antigen: DYKDDDDK Tag
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Clone: FG4R
• Conjugate: Unconjugated
• Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
• Immunogen: Synthetic peptide (DYKDDDDK) coupled to KLH.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Tag