Test
Combines affinity chromatography with desalting for the purification of human antibodies
Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.
Content And Storage | 4°C to 30°C, 20% Ethanol |
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For Use With (Equipment) | For syringe, peristaltic pump or a chromatography system |
Binding Capacity | ≈50 mg Human IgG/mL |
For Use With (Application) | Chromatography System, Manual |
Protein A conjugated with Horseradish peroxidase for detection of immunoglobulins via affinity to the Fc region - typically IgG from many mammalian species
Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.
Content And Storage | 4°C to 8°C, 20% Ethanol |
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For Use With (Equipment) | For syringe, peristaltic pump or a chromatography system |
Binding Capacity | 20 mg/mL IgG Medium |
For Use With (Application) | Chromatography System, Manual |
For simple, small-scale antibody purification or enrichment of specific proteins by antibody capture in single-use spin columns
Combines affinity with size exclusion chromatography to achieve very high purity of human IgG
Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.
Content And Storage | 4°C to 30°C |
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Description | HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat. |
Storage Buffer | 20% Ethanol |
Product Type | Pre-packed Columns |
For Use With (Application) | Designed for use with a syringe, peristaltic pump, or chromatography system |
For small-scale purification of antibodies or enrichment of proteins of interest by antibody capture in 96-well filter plates
Pre-made buffers optimized for the affinity-based enrichment of a target protein/immunoprecipitation using Protein A HP, Protein G HP, and Ab SpinTrap™ columns
MabSelect PrismA LigandRestricted Licens