Test
Used for the optimization of transfection and verification of editing efficiency in transfected cells.
Ambion™ Carrier RNA (1 mg/mL) is a component of all the MagMAX™ Isolation Kits that may be purchased separately.
Non-targeting gRNA sequences that do not recognize any sequence in the human or mouse genome.
Superior quality GeneArt CRISPR Nuclease mRNA is formulated to improve product performance.
Provides an efficient target for antiviral agents.
Ready to use RNase-free solution with low toxicity to use with live cells for determination of RNA structure
Used for the optimization of transfection and verification of editing efficiency in transfected cells.
Ready-to-use, synthetic gRNAs maximizes and simplifies the performance of genome editing experiments.
Used for the optimization of transfection and verification of editing efficiency in transfected cells.
The EnGen sgRNA Synthesis Kit, S. pyogenes provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.
Yeast 5' Deadenylase is a member of the HIT (histidine triad) family proteins and specifically a member of the Hint branch. It is the yeast orthologue of aprataxin. Mutations in human aprataxin have been known to be involved in the neurological disorder known as ataxia oculomotor apraxia-1. The human protein has been shown to resolve abortive ligation intermediates by removing the AMP at the 5 end of DNA (AMP-DNA hydrolase activity) . It also repairs DNA damage at 3 ends by removing 3-phosphate and 3-phosphoglycolate. Human aprataxin acts on small molecules as well, nucleotide polyphosphates such as diadenosine tetraphosphate (AppppA) as well as lysyl-AMP. The 5' Deadenylase is encoded by HNT3 gene of S. cerevisiae. NEB has shown this protein is capable of deadenylation from 5 end of DNA and RNA, leaving the phosphate at 5' end. It also cleaves AppppA into ATP and AMP. Its activity on lysyl-AMP is not detectable.
The EnGen sgRNA Synthesis Kit, S. pyogenes provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue fluorescent CF350 dye has excitation/emission at 347/448 nm.
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF660R dye has excitation/emission at 663/682 nm.
T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L). This product is packaged with a vial of 100 mM ATP (N0451A). The increased concentration of reaction components is helpful in lower volume reactions where reagent volumes are limited. T4 RNA Ligase 1 catalyzes the ligation of a 5 phosphoryl-terminated nucleic acid donor to a 3 hydroxyl-terminated nucleic acceptor through the formation of a 3 5 phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include singlestranded RNA and DNA as well as dinucleoside pyrophosphates.