Test
Consists of five different plasmids, each of which is designed to express multiple molecular chaperones that function as a "chaperone team" to enable protein folding. Co-expression of a target protein with one of these chaperone teams increases the recovery of soluble proteins. Each plasmid carries an origin of replication derived from pACYC and a Cmr gene, which allows use with E. coli expression systems utilizing ColE1-type plasmids containing an ampicillin resistance gene as a marker. The chaperone genes are situated downstream of an araB or Pzt-1 (tet) promoter. Therefore, expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters (e.g. lac). These plasmids also contain the necessary regulator (araC or tetr) for each promoter. 1 Set
The only difference is that each contains either 100% unmodified cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines. Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine modifications.
The Zyppy-96 Plasmid Miniprep allows efficient isolation of plasmid DNA from E. coli, featuring a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
The Human HCT116 DKO Non-methylated DNA is purified from cells that contain genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-).The DNA derived from HCT116 DKO cells has a low level of DNA methylation and can be used as a control for DNA methylation analysis. The Human HCT116 DKO Methylated DNA is purified HCT116 DKO DNA that has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by M.SssI methyltransferase2 (EC 2.1.1.37) and can be used as a positive control for DNA methylation analysis.
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process - simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 l. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
Calcium Chloride 2 M is useful in the preparation of competent E.coli and transformation of bacteria.
Over the past few years, SNAP-Tag technology combined with TR-FRET has paved the way for the development of many non-radioactive, no-wash, binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag and subsequently labeling them with Terbium. Cisbio offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready for protein expression and labeling. All information on this page pertains to the Tag-lite plasmid cloned with the Beta2 adreno receptor.
Over the past few years, SNAP-Tag technology combined with TR-FRET has paved the way for the development of many non-radioactive, no-wash, binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag and subsequently labeling them with Terbium. Cisbio offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready for protein expression and labeling. All information on this page pertains to the Tag-lite plasmid cloned with the OX2 Orexin receptor.
For use in E. coli mutagenicity test with E. coli WP2 tester strains. Dose 2.0 µL/plate. This product comes in neat format. Add organic solvents such as DMSO or sterile dH2O as applicable. Direct acting mutagens do not require the use of S9 metabolic activation to cause mutagenicity. CONTROLCHEM™ chemicals are obtained from major suppliers of research chemicals and are employed without further characterization or purification. Packaged quantities are precise within 1%. Please Note: CONTROLCHEM™ chemicals are known mutagens/carcinogens/toxins and are sold only to those experienced in the safe handling and disposal of hazardous materials. Consult your Institutional Safety Officer before ordering.
Over the past few years, SNAP-Tag technology combined with TR-FRET has paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag and subsequently labeling them with Terbium. PT8HALOZEO is a backbone containing the gene for the HALO tag, a promoter a zeomycin resistance gene and MCS (Multiple Cloning Site).
Over the past few years, SNAP-Tag technology combined with TR-FRET has paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag and subsequently labeling them with Terbium. PT8CLIPZEO is a backbone containing the gene for the CLIP tag, a promoter, a zeomycin resistance gene and MCS (Multiple Cloning Site).
Over the past few years, SNAP-Tag technology combined with TR-FRET has paved the way for the development of many non-radioactive, no-wash, binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag and subsequently labeling them with Terbium. Cisbio offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready for protein expression and labeling. All information on this page pertains to the Tag-lite plasmid cloned with the GLP1 Glucagon receptor.
For absorbance-based quantitation of global DNA methylation in an ELISA-like format
The pLenti-GFP Lentiviral Control Vector may be co-transfected with third-generation lentiviral packaging constructs to make a lentiviral particle containing GFP. The resulting lentivirus is useful as a transduction control.
634889 \ SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing \ 24 Rxns