Test
Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform
Trap-Pak™ bags are tightly sealed membrane packets containing molecular sieves to reduce and maintain a low water content in solutions that must remain anhydrous.
High-purity enzyme that's a mix of cloned RNA polymerase and RNase inhibitor
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
Promoter | T7 |
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Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TA Cloning |
Bacterial or Yeast Strain | Not Included |
For Use With (Application) | PCR Cloning |
Vector | pCR2.1 |
Product Line | TA Cloning™ |
Provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli is available in 20- and 40-reaction kit sizes.
Promoter | T7 |
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Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TA Cloning |
Bacterial or Yeast Strain | TOP10 |
For Use With (Application) | PCR Cloning |
Product Line | One Shot™ |
Cell Type | Chemically Competent E. coli |
VetMAX™ Xeno™ Internal Positive Control (IPC) RNA serves as an internal positive control for the RNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as oral fluids.
Shipping Condition | Dry Ice |
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Includes | Internal Positive Control |
Format | Kit |
Product Type | Internal Positive Control RNA |
For Use With (Application) | Detection and Identification of Animal Disease |
Species | Not Species-Specific |
Product Line | VetMAX™, Xeno™ |
A versatile and efficient tool for introducing point mutations, insertions, or deletions into any type of plasmid DNA. With this kit the entire plasmid is amplified using phosphorylated primers that introduce the desired changes.
For fast, efficient cloning and subsequent in vitro transcription/translation
Promoter | T7, SP6 |
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Content And Storage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPO™-TA |
Bacterial or Yeast Strain | TOP10 |
Cell Type | Chemically Competent |
Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.
Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing
Promoter | T7, T3 |
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Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPO™ |
Bacterial or Yeast Strain | TOP10 |
For Use With (Application) | Chromatin Biology |
Vector | Blunt DNA Cloning Vectors |
Product Line | One Shot™ |
Cell Type | Chemically Competent |
Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPO™ |
Bacterial or Yeast Strain | TOP10 |
Cell Type | Chemically Competent |
Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)
Shipping Condition | Approved for shipment at Room Temperature or on Wet or Dry Ice |
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Promoter | T3, T7 |
Content And Storage | Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes Box 3: • One Shot OmniMAX 2 T1R chemically competent E. coli • S.O.C. Medium • pUC 19 Control DNA Store competent cells and pUC19 at -65 to -85°C. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | Zero Blunt TOPO |
Bacterial or Yeast Strain | OmniMAX2 |
For Use With (Application) | PCR Cloning |
Vector | pCR-XL-2-TOPO |
Product Line | TOPO™ |
Cell Type | Chemically Competent E. coli |
Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases
Promoter | T7 |
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Content And Storage | Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt PCR |
Bacterial or Yeast Strain | Not Included |
Vector | Blunt DNA Cloning Vectors |
Product Line | TOPO™, Zero Blunt™ |