HPLC Workflow: Let’s Get Started

Have dirty samples? Working with molecules that are difficult to separate? Are you tackling a new HPLC application? Finding the right HPLC products and technical support is the first step in optimizing your HPLC workflow and achieving successful separations.

You'll find what you need right here: With tools for sample preparation, ultrapure water, and a complete range of chromatography resins and solvents, MilliporeSigma has solutions for every phase of your HPLC workflow.


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Sample Preparation

As HPLC becomes faster and more sensitive, sample preparation is also becoming faster and easier to perform. From Millex™ Syringe Filters, available in a range of sizes, to the Samplicity™ vacuum-driven Filtration System for hard-to-filter samples, MilliporeSigma products let you spend more time gathering data and finding answers and less time struggling with sample preparation.


Stationary Phase

From the simplest to the most challenging analyses, MilliporeSigma HPLC Columns deliver clean, reliable, reproducible separations. And they’re backed by a team of experts who stand ready to provide you with technical and application support.


Mobile Phase

Mobile Phases for Optimized Separations
High-performance liquid chromatography requires high-performance solvents. Don't compromise on the mobile phase — use MilliporeSigma Solvents, which meet the highest-quality standards and ensure successful HPLC. And for your integral pure and ultrapure water needs, Millipore Sigma offers a broad range of water purification systems.

Cell to Protein

Prepare, Grow, Purify, Analyze

Discover more with EMD Millipore — convenient, reliable solutions to support your research from preparation to analysis.


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Prepare your cell culture media with sterile filters from EMD Millipore. With innovative filtration devices and more than 50 years of membrane technology expertise, EMD Millipore offers a wide range of options to make each critical step quick, easy and accurate.



Our MultiScreen™ and Millicell™ Plates and Inserts provide maximum user convenience and reliability for cell culture. With them, cell growth, structure and function more closely replicate in vivoconditions. And the Millicell™ EZ SLIDE brings enhanced features and a unique design that helps you spend less time dealing with clumsy devices and more time on your all-important cell culture research.



EMD Millipore supports every step of your sample preparation, from protein extraction and purification to concentration and desalting, offering a wide selection of ultrafiltration devices, magnetic beads and more.

Western Blotting

Western Blotting

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. EMD Millipore has helped scientists publish their Western blots for decades by providing continuous innovation and steadfast technical support.


EMD Millipore Protein Blotting Handbook Request

Tips and tricks to help you with your Westerns. Get your copy today!

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Protein Preparation

Why spend your time on arduous sample preparation protocols when you can focus your efforts on exciting experiments? Purify and concentrate your proteins more efficiently, and your next discovery is only a step away.


Transfer Membranes

EMD Millipore offers three different PVDF membranes, each optimized for different protein blotting applications. Or try blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.



EMD Millipore Reagents are precisely developed and produced to give you reliable and reproducible results across all your applications.

  • Bløk™: Ready-to-use, chemical-based, protein-free blocker that removes background risk
  • Luminata™: Premixed HRP substrates that offer the right sensitivity at the right price
  • Reblot™: Room-temperature stripping in 15 minutes without the smell
Sample Filtration

Samplicity G2 Filtration System for HPLC Samples

The Better Way to Use Millex Filters

For chromatographers who filter dozens of HPLC samples a day through Millex™ syringe filters, especially for hard-to-filter, viscous or particulate-laden samples, traditional filtration can lead to manual fatigue. Nevertheless, they stick with Millex™ filters, which are specified in numerous HPLC methods and standard operating procedures. The Samplicity™ G2 multi-sample filtration system enables you to enjoy Millex™ quality, but skip the pain of manual syringe filtration.

The newest Samplicity™ G2 system gives you:

  • Vacuum filtration of 1-8 samples in seconds directly into HPLC vials
  • An ergonomic alternative to syringe filters
  • Broader membrane selection
  • Compatibility with any protocol or SOP specifying 33 mm Millex™ filters

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Millex Syringe Filters

Millex™ syringe filters set the standard for reliable small-volume sterile filtration.

  • Low Extractables, Low Binding 
    A variety of membranes and housings ensure compatibility with a range of solutions
  • Faster Flow Rate 
    33mm Millex filters have 20% more filter surface area for significantly higher flow rate and throughput
  • Low Hold-up Volume 
    Despite the increased membrane area in the 33mm Millex filters, the hold-up volume (after air purge) has been reduced to 80µL, a 20% decrease over 25mm filters
  • Higher Operating Pressure 
    Millex 33mm filters can withstand maximum housing pressure of 150 psig (10-bar) so that solutions filter faster


Fuel, Hydraulic Fluids and Machined Parts

The machines that keep global industries running need clean parts, fuel and hydraulic fluids that are free of particles and other contamination.

Environmental Monitoring and Analysis

Whether you need to collect air to monitor particulate contamination, or analyze soil or water for hazardous chemicals, MilliporeSigma can supply the expertise, products and protocols to ensure results that are compliant with regulatory requirements. MilliporeSigma products designed for environmental applications have been developed for use with standard methods, including those of NIOSH, OSHA and ASTM.


Air Monitoring

Particle contamination is a concern during the processing, manufacturing and packaging of pharmaceuticals, foods and mechanical and electrical components. MilliporeSigma has applied its extensive filtration expertise to a broad range of products optimized to help you comply with ever-decreasing detection limits in occupational and environmental monitoring.


Soil Monitoring

MilliporeSigma offers a Zero Head Space Extractor system for sample extraction that meets the requirements of the EPA's Toxicity Characteristic Leaching Procedure (TCLP), plus a variety of filters, holders and manifolds optimized to deliver superior results during downstream analyses.


Water Testing

The MilliporeSigma family of certified sample preparation devices provide a disposable, yet reliable method for collecting and preparing environmental samples for downstream analysis. From groundwater collection to chromatographic analysis, there is a device to simplify your procedure and improve your results.



Filtration of Aqueous and Organic Solutions

Whether you need to separate or remove contaminants from air or fluid samples prior to downstream analysis, MilliporeSigma's broad selection of membrane types and pore sizes ensure that the right filter is available for your application. Membrane pore sizes range from 0.025µm to 12µm and net filters (nylon or polypropylene) are available from 11µm to 180µm. Filter discs are available in 13mm to 293mm. Non-sterile Millex syringe filters are available in a new, larger 33mm housing with MilliporeSigma Express PLUS (PES), Durapore (PVDF) and nylon membranes.


MilliporeSigma Multiscreen (Multiwell Plate Filtration)

We offer hundreds of plate-based products for filtration and cell growth including numerous plate accessories. Applications for Multiscreen plates include:

  • Biochemistry
  • Personalized Medicine
  • Molecular Biology
  • Sample Prep
  • Drug Safety Analysis
  • Mammalian Cell Culture
  • Cell-Based Assays

Small-Scale BioProcessing Products - Filtration and Purification

MilliporeSigma offers small-scale bioprocessing products enabling you to conduct your own process development using proven, reliable consistent and scalable devices. Our product offering includes devices for prefiltration, clarification, ultrafiltration, sterile filtration, chromatography and vent filtration for use in vaccine, biotech and classical pharmaceutical process applications.

Water Purification

As the most commonly used solvent, your laboratory relies heavily on the quality of your water.

MilliporeSigma offers solutions for: Type 1 - Ultrapure, Type 2 - Pure, Type 3 - RiOS


Type 1 - Ultrapure

Several MilliporeSigma water systems are designed to produce ultrapure (Type 1) water. Some can operate directly from tap (potable) water and others from pure water (Elix, RiOs, distilled or DI water).

The selection of the proper ultrapure water system for your laboratory will depend on several parameters such as: feed water available in the laboratory, instant and daily volume needs of ultrapure water or ultrapure and pure water, the quality of monitoring of water quality and the level of certification required and several other points.


Type 2 - Pure

Several MilliporeSigma water systems are designed to produce pure water (Type 2), combining several purification technologies suitable for applications like buffer preparation, pH solution preparation and microbiological media preparation and feed to laboratory systems.

From tap to pure:


Type 3 - RiOS

The production of pure water is indispensable in any laboratory. However, for most users, the production of pure water involves high costs and extensive maintenance due to inefficient or outdated water purification systems. Furthermore, most water purification systems cannot guarantee the production of consistently pure water quality.

Several MilliporeSigma water systems are designed to produce pure water (Type 3) combining several purification technologies suitable for applications that feed to: washing machines for final rinsing of glassware, heating baths, autoclaves, etc.

From tap to pure:


Chemicals, Analytics, Chromatography

At MilliporeSigma, we believe that quality in means quality out. That's why our reagents and chemicals are subjected to stringent controls during development and production to ensure reliable and reproducible results. Whether for analysis or synthesis, complex or routine applications, research or production or even cleaning or monitoring, we give you a choice of more than 30,000 premium chemicals and reagents to facilitate your daily work. And, should you need further support, you can expect the same excellent quality from our service teams around the world.