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MilliporeSigma™ Upstate Protein A Agarose/Salmon Sperm DNA

Blocks non-specific DNA binding sites on agarose beads.

Supplier:  MilliporeSigma™ 16157

Catalog No. 16-157


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Description

Description

EMD Millipore Upstate Protein A Agarose/Salmon Sperm is covalently bound to agarose beads for use in chromatin immunoprecipitations.

  • Protein A covalently bound to agarose by alkylamine linkage
  • Protein A from Staphylococcus aureus has very high affinity for Fc regions of IgG molecules
  • Immobilization of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media
  • Qualified using Acetyl Histone H4 H3 ChIP assay
  • Suitable for use in chromatin immunoprecipitation (ChIPs) assays
Specifications

Specifications

Agarose/Salmon Sperm DNA
Chromatin immunoprecipitation (ChIPs) assays
4°C
10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% Sodium Azide.
2.5mL packed beads
2.5 mL
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