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MilliporeSigma™ Upstate Protein A Agarose/Salmon Sperm DNA
Blocks non-specific DNA binding sites on agarose beads.
Supplier: MilliporeSigma™ 16157
Description
EMD Millipore Upstate Protein A Agarose/Salmon Sperm is covalently bound to agarose beads for use in chromatin immunoprecipitations.
- Protein A covalently bound to agarose by alkylamine linkage
- Protein A from Staphylococcus aureus has very high affinity for Fc regions of IgG molecules
- Immobilization of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media
- Qualified using Acetyl Histone H4 H3 ChIP assay
- Suitable for use in chromatin immunoprecipitation (ChIPs) assays
Specifications
Agarose/Salmon Sperm DNA | |
Chromatin immunoprecipitation (ChIPs) assays | |
4°C |
10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% Sodium Azide. | |
2.5mL packed beads | |
2.5 mL |
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