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Invitrogen™ Total Nitric Oxide Assay Kit

Measure total nitric oxide (NO) as the single value (sum) of nitrate (NO3-) and nitrite (NO2-) levels in serum, plasma, urine, saliva and other biological fluids.

Supplier:  Invitrogen™ EMSNOTOT

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Catalog No. ENEMSNOTOT


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Description

Description

Assay total nitric oxide (NO) as the single value (sum) of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Total Nitric Oxide Assay Kit.
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Nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. Total nitric oxide (NO) contributed by nitrate and nitrite in a system is measured as nitrite after converting all nitrate to nitrite.

  • Target: Total nitric oxide (NO) contributed by the sum of nitrite (NO2-) and nitrate (NO3-)

  • Format: Colorimetric stain (azo dye) for clear 96-well plates

  • Measurement: Absorbance at 540nm (±20nm)

  • Assay Range: 3.1 to 100μM

  • Sensitivity: 1.35μM

  • Precision: Dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)

  • Sample Types: Urine, saliva, plasma, serum and other biological fluids

  • Total Assay Time: 45 minutes

  • Sample Size: 50μL

Includes:Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.

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