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Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

$482.00 - $888.00


Content And Storage Store at 4°C.
Product Line B-PER™
Formulation Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
Product Type Bacterial Protein Extraction Reagent
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Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
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Thermo Scientific™
250 mL
Each for $482.00
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Thermo Scientific™
500 mL
Each for $888.00
Add to Cart


  • An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
  • All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
  • This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
  • Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features of the B-PER Bacterial Protein Extraction Reagent:

  • Detergent in Tris buffer with Lysozyme and Universal Nuclease
  • Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
  • Recovery of large molecular weight proteins (high MW proteins)
  • Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
  • More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications


Store at 4°C.
Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
Bacterial Protein Extraction Reagent


Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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