Thermo Scientific™ Phusion Green HF Buffer Pack

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Supplier:  Thermo Scientific™ F538L

Catalog No. F538L

Includes: Phusion HF Buffer Pack

• 5x Phusion HF Buffer (1X buffer provides 1.5 mM MgCl₂)
• 50 mM MgCl₂ solution
• DMSO

Phusion Green HF Buffer Pack

• 5X Phusion Green HF Buffer (1X buffer provides 1.5 mM MgCl₂)
• 50 mM MgCl₂ solution
• DMSO

Description

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:
• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Related Products
Phusion HF Buffer Pack (Cat. No. F518L)
Phusion HF Buffer Pack, detergent-free (F520L)
Phusion GC Buffer Pack (F519L)
Phusion GC Buffer Pack, detergent-free (F521L)
Phusion Green GC Buffer Pack (F539L)

Specifications

• Content And Storage: • 5X Phusion Green HF Reaction Buffer (4 x 1.5 mL)
  • 50 mM MgCl₂ (4 x 1.5 mL)
  • DMSO (0.5 mL)
  
  Store at –20°C.
• Shipping Condition: Wet or Dry Ice
• Starting Material: DNA

• Polymerase: Phusion High-Fidelity DNA Polymerase
• For Use With (Application): Standard PCR, High-fidelity PCR
• Volume: 4 x 1.5 mL

For Research Use Only. Not for use in diagnostic procedures.