Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ EpiJET Bisulfite Conversion Kit

The EpiJET Bisulfite Conversion Kit is used for an efficient bisulfite modification of DNA for methylation analysis.

Supplier:  Thermo Scientific™ K1461

Catalog No. FERK1461


Add to Cart

Description

Description

Thermo Scientific EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged. Bisulfite converted unmethylated cytosines are detected as thymines in the following PCR.

The EpiJET Bisulfite Conversion Kit integrates the thermal DNA denaturation and the bisulfite conversion reactions into one step. This step can be performed following long or short protocol, depending on the downstream application of modified DNA. The converted DNA is when bound to a membrane of a micro column for on-column desulfonation and subsequent DNA purification steps. The converted DNA is eluted in a small volume of Elution Buffer and is suitable for a number of techniques used for the methylation status analysis, including PCR, qPCR, COBRA, sequencing.

Highlights
• High conversion efficiency and specificity (≥99%)
• Flexibility, provided by short DNA conversion protocol
• Pure DNA, suitable for downstream applications and long-term storage

Applications
Converted DNA can be used in:
• PCR
• qPCR
• Combined Bisulfite Restriction Analysis (COBRA)
• Sequencing

Includes
• Modification Reagent
• Modification Solution I
• Modification Solution II
• Binding Buffer for Bisulfite Conversion Kit
• Wash buffer (conc.) for Bisulfite Conversion Kit
• Desulfonation Buffer (conc.)
• Elution Buffer
• DNA Purification Micro Columns & Collection Tubes

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Bisulfite Conversion Kit
EpiJET™
Product Suggestions

Product Suggestions

SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.