Applied Biosystems™ TaqMan™ Array Human Stem Cell Pluripotency

TaqMan™ Array Plates contain the same high quality TaqMan™ Gene Expression Assays that have been commercially available for years in a convenient, dried, 96-well format.

Supplier:  Applied Biosystems™ 4414077

Catalog No. 44-140-77

Description

The assay panel in this plate was selected in cooperation with the International Stem Cell Initiative (2007 Nature Biotechnology. 25:803-816). The assays target genes which are validated as markers for the characterization of human embryonic stem cell identity, and which assess variations between embryonic stem cell isolates. Genes are included based on the following criteria: Expression in human embryonal carcinoma cells, primordial germ cells or human embryonic stem cells; Known role in maintenance of pluripotency in human embryonic stem cells; Identification in microarray-based experiments as being correlated with the stem cell state. In order to identify cells which have left the pluripotent state, genes whose expression is characteristic of specific differentiation lineages in the post implantation embryo and mature tissues are included.
Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.
TaqMan Array Plates contain the same TaqMan Gene Expression Assays that have been available for years in a dried, 96-well format. Tests have found no difference in performance between TaqMan Assays supplied in the liquid format and assays dried into a TaqMan Array Plate.

• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
• Fast delivery; on your bench in days
• Convenient; dried PCR primer and TaqMan™ probe sets supplied in a 96- well plate; just add master mix and your sample
• TaqMan Array Plates have been optimized for use with TaqMan Gene Expression Master Mix, but are also compatible with TaqMan Universal PCR Master Mix
• For studies using cultured cells, TaqMan Array Plates are also compatible (and recommended) with the TaqMan Gene Expression Cells-to-Ct™ Kit
• TaqMan Array Plates must be run on a real-time PCR instrument with 96-well plate capability
• The plates have been validated for use on Applied Biosystems™ 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20μL reaction
• Each pack of TaqMan Array Plates is shipped with a CD containing the plate map along with details of each TaqMan Assay enabling proper labeling of data and easy access to Assay ID numbers for simple reordering

Specifications

• Content And Storage: Each TaqMan™ Array Plate well contains a single TaqMan™ Assay for one 20 μL reaction. The plates can be stored at room temperature or at 4°C.
• Format: 96-well plate
• Internal Probe Modification: MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')
• Product Line: TaqMan™
• Research Category: Stem Cell Research, Reproduction, Development
• For Use With (Application): Gene Expression

• Form: Dried Down
• Reaction Speed: Standard
• Label or Dye: FAM
• Purification Method: Standard
• Species: Human
• No. of Reactions: 1 x 20 μL Reactions

For Research Use Only. Not for use in diagnostic procedures.