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Gibco™ RevitaCell™ Supplement (100X)

RevitaCell™ Supplement (100X)

Supplier:  Gibco™ A2644501

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Catalog No. A2644501


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Description

Description

RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.

  • Enabling—provides maximum cell viability when performing single cell passaging
  • Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons
  • Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence

    Single cell passaging
    Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.

    Higher specificity
    Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).

    Quicker post-thaw recovery
    PSCs recovered at 40,000 viable cells/cm2 using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.
    TRUSTED_SUSTAINABILITY
    • Specifications

    Specifications

    Store at -5 to -20°C and protect from light.
    Animal Origin-free
    Stem Cell (Embryonic, iPS - Induced Pluripotent Stem)
    cGMP for medical devices, 21 CFR Part 820 and ISO 13485
    Dry Ice
    Stem Cells (iPS - Induced Pluripotent Stem)
    100 X
    Media Supplement
    Gibco™, RevitaCell™
    Animal origin-free
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