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Promega Riboprobe™ Combination Systems
The Riboprobe Systems are designed to prepare high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.
Supplier: Promega P1460
Includes: RNA polymerase(s); pGEM™ Express Positive Control Template; RQ1 RNase-free DNase; Recombinant RNasin™ Ribonuclease Inhibitor; rATP; rCTP; rGTP; rUTP; DTT; Transcription-optimized buffer, 5X; Nuclease-free water
Description
- Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts
- Choose from combination systems with T3/T7 or SP6/T7 RNA polymerases
- SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
- Allows synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct
Specifications
| -30°C to -10°C | |
| 500U SP6 RNA Polymerase, 500U T7 RNA Polymerase, 5μg pGEM™ express positive control template, 500μL transcription optimized 5X Buffer, 2500U recombinant RNasin™ R | |
| 1 System |
| Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA | |
| Riboprobe Combination System SP6/T7 |
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